Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
Product Information
Format
Culture Supernatant
Control
HeLa cell lysate, treated with colcemid.
Presentation
Supplied as 200 μL of culture supernatant with 0.05% sodium azide.
Applications
Application
Use Anti-phospho-Histone H1 Antibody, clone 12D11 (Mouse Monoclonal Antibody) validated in IHC, ICC, IP, WB to detect phospho-Histone H1 also known as Histone H1a, histone 1 H1b.
Key Applications
Immunohistochemistry
Western Blotting
Immunoprecipitation
Immunocytochemistry
Application Notes
Immunohistochemistry: This antibody has been reported by an independent laboratory to detect CDK (cyclin dependent kinase) phosphorylated Histone H1 in benign tissue sections.1
Immunocytochemistry:
This antibody has been demonstrated by an outside laboratory to show positive immunostaining for phosphorylated histone H1 in HUSK (human fetal primary myoblasts) cells fixed with 3.7% paraformaldehyde followed by permeabilization with 0.2% Triton X-100.2
Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to immunoprecipitate phosphorylated histone H1 using solid phase beads bound to anti-mouse IgM.
Biological Information
Immunogen
The immunogen was KLH-conjugated bovine nuclear extract.
Clone
12D11
Host
Mouse
Specificity
Recognizes phosphorylated Histone H1, Mr 32 kDa.
Isotype
IgMκ
Species Reactivity
Human Vertebrates Mouse Bovine
Species Reactivity Note
Human, mouse, bovine. Broad species cross-reactivity is expected, based on sequence homology.
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is
wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq].
FUNCTION:Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY:Belongs to the histone H1/H5 family.
Product Usage Statements
Quality Assurance
Western Blot Analysis:
1:1000 dilution of this antibody detected Histone H1 in colcemid-treated HeLa lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size
200 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, Vrt, M, B
IHC, WB, IP, ICC
M
Culture Supernatant
Monoclonal Antibody
Description
Catalogue Number
05-1324
Description
Anti-phospho-Histone H1 Antibody, clone 12D11
Alternate Names
H1 histone family, member 5
H1b
histone cluster 1
H1b
histone 1
Histone H1a
Background Information
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.
Product Information
Format
Culture Supernatant
Control
HeLa cell lysate, treated with colcemid.
Presentation
Supplied as 200 μL of culture supernatant with 0.05% sodium azide.
Applications
Application
Use Anti-phospho-Histone H1 Antibody, clone 12D11 (Mouse Monoclonal Antibody) validated in IHC, ICC, IP, WB to detect phospho-Histone H1 also known as Histone H1a, histone 1 H1b.
Key Applications
Immunohistochemistry
Western Blotting
Immunoprecipitation
Immunocytochemistry
Application Notes
Immunohistochemistry: This antibody has been reported by an independent laboratory to detect CDK (cyclin dependent kinase) phosphorylated Histone H1 in benign tissue sections.1
Immunocytochemistry:
This antibody has been demonstrated by an outside laboratory to show positive immunostaining for phosphorylated histone H1 in HUSK (human fetal primary myoblasts) cells fixed with 3.7% paraformaldehyde followed by permeabilization with 0.2% Triton X-100.2
Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to immunoprecipitate phosphorylated histone H1 using solid phase beads bound to anti-mouse IgM.
Biological Information
Immunogen
The immunogen was KLH-conjugated bovine nuclear extract.
Clone
12D11
Host
Mouse
Specificity
Recognizes phosphorylated Histone H1, Mr 32 kDa.
Isotype
IgMκ
Species Reactivity
Human Vertebrates Mouse Bovine
Species Reactivity Note
Human, mouse, bovine. Broad species cross-reactivity is expected, based on sequence homology.
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is
wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq].
FUNCTION:Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY:Belongs to the histone H1/H5 family.
Product Usage Statements
Quality Assurance
Western Blot Analysis:
1:1000 dilution of this antibody detected Histone H1 in colcemid-treated HeLa lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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