B lymphoma Mo-MLV insertion region 1 homolog (mouse)
Background Information
The Bmi-1 protein is a member of the polycomb group (PcG) proteins. Bmi-1 has been reported as an oncogene by regulating the cell cycle inhibitors p16 and p19. Bmi-1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi-1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Bmi-1 may regulate the self-renewal of other types of somatic stem cells, and it is also thought to inhibit aging in neurons through the suppression of p53. Several lines of evidence implicate Bmi-1 in tumorigenesis. Differential expression of Bmi-1 in a mouse model of metastatic prostate cancer vs. wild-type suggests Bmi-1 expression levels may predict response to therapy and survival in multiple types of cancer.
Product Information
Format
Ascites
Control
U20S Cell Lysate
Presentation
Mouse monoclonal IgG1κ ascites in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-Bmi-1 Antibody, clone DC9 (Mouse Monoclonal Antibody) validated in WB, IP to detect Bmi-1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to be suitable for immunoprecipitation1.
Biological Information
Immunogen
Recombinant fusion protein of full length murine BMI-1 tagged with MBP.
Clone
DC9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Ref.8 Ref.11
SUBUNIT STRUCTURE:Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Ref.11 Ref.4 Ref.5 Ref.7 Ref.9
SUBCELLULAR LOCATION:Nucleus. Cytoplasm. Ref.11
PTM:May be polyubiquitinated; which does not lead to proteasomal degradation.
DISEASE:Cooperates with the MYC oncogene to produce B-lymphomas.
SIMILARITY:Contains 1 RING-type zinc finger.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on U20S cell lysate.
Western Blot Analysis:
1:5,000 of this lot detected BMI-1 on 15 μg/mL of U20S cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, M
WB, IP
M
Ascites
Monoclonal Antibody
Description
Catalogue Number
05-1322
Description
Anti-Bmi-1 Antibody, clone DC9
Alternate Names
B lymphoma Mo-MLV insertion region 1 homolog
polycomb group ring finger 4
oncogene BMI-1
murine leukemia viral (bmi-1) oncogene homolog
RING finger protein 51
Polycomb group RING finger protein 4
BMI1 polycomb ring finger oncogene
B lymphoma Mo-MLV insertion region 1 homolog (mouse)
Background Information
The Bmi-1 protein is a member of the polycomb group (PcG) proteins. Bmi-1 has been reported as an oncogene by regulating the cell cycle inhibitors p16 and p19. Bmi-1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi-1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Bmi-1 may regulate the self-renewal of other types of somatic stem cells, and it is also thought to inhibit aging in neurons through the suppression of p53. Several lines of evidence implicate Bmi-1 in tumorigenesis. Differential expression of Bmi-1 in a mouse model of metastatic prostate cancer vs. wild-type suggests Bmi-1 expression levels may predict response to therapy and survival in multiple types of cancer.
Product Information
Format
Ascites
Control
U20S Cell Lysate
Presentation
Mouse monoclonal IgG1κ ascites in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-Bmi-1 Antibody, clone DC9 (Mouse Monoclonal Antibody) validated in WB, IP to detect Bmi-1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to be suitable for immunoprecipitation1.
Biological Information
Immunogen
Recombinant fusion protein of full length murine BMI-1 tagged with MBP.
Clone
DC9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Ref.8 Ref.11
SUBUNIT STRUCTURE:Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Ref.11 Ref.4 Ref.5 Ref.7 Ref.9
SUBCELLULAR LOCATION:Nucleus. Cytoplasm. Ref.11
PTM:May be polyubiquitinated; which does not lead to proteasomal degradation.
DISEASE:Cooperates with the MYC oncogene to produce B-lymphomas.
SIMILARITY:Contains 1 RING-type zinc finger.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on U20S cell lysate.
Western Blot Analysis:
1:5,000 of this lot detected BMI-1 on 15 μg/mL of U20S cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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