SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. This protein is component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-SUZ12 Antibody, clone 2AO9 (Mouse Monoclonal Antibody) validated in IP, ICC to detect SUZ12 also known as ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein.
Key Applications
Immunoprecipitation
Chromatin Immunoprecipitation (ChIP)
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.
Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChiP. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.
Biological Information
Immunogen
Recombinant protein corresponding to human SUZ12.
Epitope
Unknown
Clone
2AO9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes SUZ12.
Isotype
IgG
Species Reactivity
Human Rabbit
Species Reactivity Note
Demonstrated to react with human. Predicted to react with rabbit based on 100% sequence homology.
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq]
FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
SIZE: 739 amino acids; 83055 Da
SUBUNIT: Component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like BMI1, CBX4, CBX8, PHC2, PHC3, SCMH1, RING1 and RNF2.
SUBCELLULAR LOCATION: Nucleus (Probable).
DISEASE: chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. & Contains 1 C2H2-type zinc finger.
Product Usage Statements
Quality Assurance
Evaluated by Immunoprecipitation in HeLa cell lysate.
Immunoprecipitation Analysis: 10 µg/mL of this antibody immunoprecipitated SUZ12 in HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, Rb
IP, ChIP, ICC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1317
Description
Anti-SUZ12 Antibody, clone 2AO9
Alternate Names
ChET 9 protein
suppressor of zeste 12 homolog (Drosophila)
joined to JAZF1
Suppressor of zeste 12 protein homolog
Joined to JAZF1 protein
Chromatin precipitated E2F target 9 protein
Background Information
SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. This protein is component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-SUZ12 Antibody, clone 2AO9 (Mouse Monoclonal Antibody) validated in IP, ICC to detect SUZ12 also known as ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein.
Key Applications
Immunoprecipitation
Chromatin Immunoprecipitation (ChIP)
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.
Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChiP. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.
Biological Information
Immunogen
Recombinant protein corresponding to human SUZ12.
Epitope
Unknown
Clone
2AO9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes SUZ12.
Isotype
IgG
Species Reactivity
Human Rabbit
Species Reactivity Note
Demonstrated to react with human. Predicted to react with rabbit based on 100% sequence homology.
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq]
FUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
SIZE: 739 amino acids; 83055 Da
SUBUNIT: Component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like BMI1, CBX4, CBX8, PHC2, PHC3, SCMH1, RING1 and RNF2.
SUBCELLULAR LOCATION: Nucleus (Probable).
DISEASE: chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. & Contains 1 C2H2-type zinc finger.
Product Usage Statements
Quality Assurance
Evaluated by Immunoprecipitation in HeLa cell lysate.
Immunoprecipitation Analysis: 10 µg/mL of this antibody immunoprecipitated SUZ12 in HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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