Poly(A) ribonuclease (PARN) is a exoribonuclease responsible for the deadenylation of mRNA. This protein is constitutively expressed in virtually all mammalian tissues. With a critical role in the post-transcriptional control of gene expression PARN consists of two RNA-binding domains and one catalytic domain. Although PARN has a preference for poly(A), data suggests the protein can also degrade poly(U).
Product Information
Format
Purified
Control
A431 cell lysate
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Applications
Application
Use Anti-PARN Antibody, clone 7A7.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect PARN also known as Poly(A)-specific ribonuclease PARN, Deadenylating nuclease.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PARN, clone 7A7.1 in HeLa cells.
Biological Information
Immunogen
Linear peptide corresponding to human PARN.
Clone
7A7.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Summary: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
FUNCTION: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
CATALYTIC SCTIVITY: Exonucleolytic cleavage of poly(A) to 5'-AMP.
COFACTOR: Divalent metal cations. Mg2+ is the most probable.
SUBUNIT STRUCTURE: Homodimer. Interacts with KHSRP and CELF1/CUGBP1. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Nucleus › nucleolus. Note: Some nuclear fraction is nucleolar.
TISSUE SPECIFICITY: Ubiquitous.
SEQUENCE SIMILARITIES: Belongs to the CAF1 family. Contains 1 R3H domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PARN on 10 µg of A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
WB, ICC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1238
Description
Anti-PARN Antibody, clone 7A7.1
Alternate Names
Poly(A)-specific ribonuclease PARN
Polyadenylate-specific ribonuclease
Deadenylation nuclease
Deadenylating nuclease
Background Information
Poly(A) ribonuclease (PARN) is a exoribonuclease responsible for the deadenylation of mRNA. This protein is constitutively expressed in virtually all mammalian tissues. With a critical role in the post-transcriptional control of gene expression PARN consists of two RNA-binding domains and one catalytic domain. Although PARN has a preference for poly(A), data suggests the protein can also degrade poly(U).
Product Information
Format
Purified
Control
A431 cell lysate
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Applications
Application
Use Anti-PARN Antibody, clone 7A7.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect PARN also known as Poly(A)-specific ribonuclease PARN, Deadenylating nuclease.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PARN, clone 7A7.1 in HeLa cells.
Biological Information
Immunogen
Linear peptide corresponding to human PARN.
Clone
7A7.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Summary: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
FUNCTION: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
CATALYTIC SCTIVITY: Exonucleolytic cleavage of poly(A) to 5'-AMP.
COFACTOR: Divalent metal cations. Mg2+ is the most probable.
SUBUNIT STRUCTURE: Homodimer. Interacts with KHSRP and CELF1/CUGBP1. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Nucleus › nucleolus. Note: Some nuclear fraction is nucleolar.
TISSUE SPECIFICITY: Ubiquitous.
SEQUENCE SIMILARITIES: Belongs to the CAF1 family. Contains 1 R3H domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PARN on 10 µg of A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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