Sulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferase enzymes in the trans-Golgi-network. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosines in the total protein content of a cell can be sulfated. Sulfotyrosines mediates many receptor-ligand binding interactions, including the interaction of P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses; for example, tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells, and tyrosine sulfation of the Duffy antigen/receptor for chemokines is crucial for erythrocyte invasion by the malaria parasitePlasmodium vivax.
This clone was derived from the antibody described in Kehoeet al., 2006.
Product Information
Format
Purified
Presentation
100 μl in 100 mM Tris, 150 mM NaCl, 180 mM Glycine, pH 7.5 containing 0.05% sodium azide.
Applications
Application
Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2 is a high quality Mouse Monoclonal Antibody for the detection of Sulfotyrosine & has been validated in WB, IP, ELISA.
Key Applications
Western Blotting
ELISA
Immunoprecipitation
Application Notes
WB, IP, ELISA
Biological Information
Immunogen
This antibody was developed using a phage display library as described in Kehoe et al., 2006. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC
Epitope
Sulfated Tyrosine
Clone
Sulfo-1C-A2
Host
Mouse
Specificity
Recognizes sulfated tyrosine residues on various proteins. Does not recognize phosphotyrosine or unmodified tyrosines.
Isotype
IgG2aκ
Species Reactivity
All
Species Reactivity Note
Broad species reactivity. Tested using human, mouse, bovine, and porcine, but the antibody is predicted to recognize any sulfotyrosine, regardless of surrounding amino acid sequence.
Antibody Type
Monoclonal Antibody
Purification Method
Culture supernatant, purified by protein G affinity chromatography
Product Usage Statements
Quality Assurance
Assayed using sulfatase treated bovine fibrinogen vs. untreated fibrinogen.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year from date of receipt when stored at +2-8°C.
Packaging Information
Material Size
30 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
A
WB, ELISA, IP
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1100X
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2
Background Information
Sulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferase enzymes in the trans-Golgi-network. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosines in the total protein content of a cell can be sulfated. Sulfotyrosines mediates many receptor-ligand binding interactions, including the interaction of P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses; for example, tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells, and tyrosine sulfation of the Duffy antigen/receptor for chemokines is crucial for erythrocyte invasion by the malaria parasitePlasmodium vivax.
This clone was derived from the antibody described in Kehoeet al., 2006.
Product Information
Format
Purified
Presentation
100 μl in 100 mM Tris, 150 mM NaCl, 180 mM Glycine, pH 7.5 containing 0.05% sodium azide.
Applications
Application
Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2 is a high quality Mouse Monoclonal Antibody for the detection of Sulfotyrosine & has been validated in WB, IP, ELISA.
Key Applications
Western Blotting
ELISA
Immunoprecipitation
Application Notes
WB, IP, ELISA
Biological Information
Immunogen
This antibody was developed using a phage display library as described in Kehoe et al., 2006. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC
Epitope
Sulfated Tyrosine
Clone
Sulfo-1C-A2
Host
Mouse
Specificity
Recognizes sulfated tyrosine residues on various proteins. Does not recognize phosphotyrosine or unmodified tyrosines.
Isotype
IgG2aκ
Species Reactivity
All
Species Reactivity Note
Broad species reactivity. Tested using human, mouse, bovine, and porcine, but the antibody is predicted to recognize any sulfotyrosine, regardless of surrounding amino acid sequence.
Antibody Type
Monoclonal Antibody
Purification Method
Culture supernatant, purified by protein G affinity chromatography
Product Usage Statements
Quality Assurance
Assayed using sulfatase treated bovine fibrinogen vs. untreated fibrinogen.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year from date of receipt when stored at +2-8°C.
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