Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).
Product Information
Format
Purified
Control
HEK293 cell lysate
Presentation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-FOXO1 Antibody, clone 2H8.2 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXO1 also known as Forkhead box protein O1A, Forkhead in rhabdomyosarcoma.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Application Notes
Immunocytochemistry: A 1:500 dilution of a previous lot was used.
Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used.
Immunohistochemistry (Paraffin): Representative lot data.
Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to full length FOXO1.
Clone
2H8.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody detects FOXO1.
FOXO1 shuttles between the nucleus and the cytosol.
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq].
FUNCTION:Transcription factor. SUBUNIT STRUCTURE:Interacts with LRPPRC By similarity. SUBCELLULAR LOCATION:Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus. TISSUE SPECIFICITY:Ubiquitous. PTM:Phosphorylated by AKT1; insulin-induced By similarity. IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. INVOLVEMENT IN DISEASE:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. SEQUENCE SIMILARITIES:Contains 1 fork-head DNA-binding domain.
Product Usage Statements
Quality Assurance
Evaluated by western blot on HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size
200 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, R, M
WB, IH(P), ICC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1075
Replaces
04-1005
Description
Anti-FOXO1 Antibody, clone 2H8.2
Alternate Names
Forkhead box protein O1A
forkhead homolog in rhabdomyosarcoma
forkhead box O1A (rhabdomyosarcoma)
forkhead box O1
Forkhead in rhabdomyosarcoma
Background Information
Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).
Product Information
Format
Purified
Control
HEK293 cell lysate
Presentation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-FOXO1 Antibody, clone 2H8.2 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXO1 also known as Forkhead box protein O1A, Forkhead in rhabdomyosarcoma.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Application Notes
Immunocytochemistry: A 1:500 dilution of a previous lot was used.
Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used.
Immunohistochemistry (Paraffin): Representative lot data.
Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to full length FOXO1.
Clone
2H8.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody detects FOXO1.
FOXO1 shuttles between the nucleus and the cytosol.
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq].
FUNCTION:Transcription factor. SUBUNIT STRUCTURE:Interacts with LRPPRC By similarity. SUBCELLULAR LOCATION:Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus. TISSUE SPECIFICITY:Ubiquitous. PTM:Phosphorylated by AKT1; insulin-induced By similarity. IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. INVOLVEMENT IN DISEASE:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. SEQUENCE SIMILARITIES:Contains 1 fork-head DNA-binding domain.
Product Usage Statements
Quality Assurance
Evaluated by western blot on HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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