PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate, as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to, and regulate mTORC1 (mTOR, Raptor, mLST8) kinase activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it to bind Raptor of mTORC1 in the absence of insulin1. It is thought that PRAS40 inhibits the kinase activity of mTORC1, when bound to Raptor, by preventing binding to its substrates such as p70S6K and 4EBP1.
Product Information
Format
Purified
Control
NIH/3T3 cell lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
This Anti-PRAS40 Antibody, clone 9D10.2 is validated for use in WB, IP, IC for the detection of PRAS40.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Western Blot Analysis: 1 µg/mL from a previous lot detected PRAS40 on 10 µg of HeLa and PC12 cell lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated PRAS40 from 100 µg of NIH/3T3 cells.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected PRAS40 in NIH/3T3 cells.
Biological Information
Immunogen
Recombinant protein corresponding to human PRAS40.
Epitope
N/A
Clone
9D10.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM].
FUNCTION: May play an important role in phosphatidylinositol 3- kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
SIZE: 256 amino acids; 27383 Da
SUBUNIT: The phosphorylated form interacts with 14-3-3.
SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (By similarity).
TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected PRAS40 on 10 µg of NIH/3T3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
M, R, H
WB, ICC, IP
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1070
Description
Anti-PRAS40 Antibody, clone 9D10.2
Alternate Names
40 kDa proline-rich AKT substrate
proline-rich Akt substrate, 40 kDa
AKT1 substrate 1 (proline-rich)
Background Information
PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate, as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to, and regulate mTORC1 (mTOR, Raptor, mLST8) kinase activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it to bind Raptor of mTORC1 in the absence of insulin1. It is thought that PRAS40 inhibits the kinase activity of mTORC1, when bound to Raptor, by preventing binding to its substrates such as p70S6K and 4EBP1.
Product Information
Format
Purified
Control
NIH/3T3 cell lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
This Anti-PRAS40 Antibody, clone 9D10.2 is validated for use in WB, IP, IC for the detection of PRAS40.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Western Blot Analysis: 1 µg/mL from a previous lot detected PRAS40 on 10 µg of HeLa and PC12 cell lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated PRAS40 from 100 µg of NIH/3T3 cells.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected PRAS40 in NIH/3T3 cells.
Biological Information
Immunogen
Recombinant protein corresponding to human PRAS40.
Epitope
N/A
Clone
9D10.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM].
FUNCTION: May play an important role in phosphatidylinositol 3- kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
SIZE: 256 amino acids; 27383 Da
SUBUNIT: The phosphorylated form interacts with 14-3-3.
SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (By similarity).
TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected PRAS40 on 10 µg of NIH/3T3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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