Insulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary agent in carbohydrate homeostasis, it has effects on fat metabolism.
Product Information
Format
Purified
Presentation
Purified mouse monolconal IgG1κ in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05%NaN3
Applications
Application
Anti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P).
Key Applications
ELISA
Immunohistochemistry (Paraffin)
Application Notes
ELISA (Direct) Analysis:
Insulin-coated plate was incubated with Anti-Insulin, clone E11D7 in 2-fold serial dilutions diluted in PBST from 5 μg/mL to 0.01 μg/mL.
Biological Information
Immunogen
Human recombinant Insulin
Epitope
Insulin
Clone
E11D7
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes insulin
Isotype
IgG1κ
Species Reactivity
Human Rat Mouse
Species Reactivity Note
Human tested. Not tested with other species, but expected to react with Mouse and Rat based on sequence homology.
After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
FUNCTION:SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. SIZE:110 amino acids; 11981 Da SUBUNIT:Heterodimer of a B chain and an A chain linked by two disulfide bonds. SUBCELLULAR LOCATION:Secreted. DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730]. SIMILARITY:SwissProt: P01308 ## Belongs to the insulin family.
Product Usage Statements
Quality Assurance
Immunohistochemistry Analysis:
20 μg/mL of this antibody detected insulin in β cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, R, M
ELISA, IH(P)
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1066
Description
Anti-Insulin Antibody, clone E11D7
Background Information
Insulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary agent in carbohydrate homeostasis, it has effects on fat metabolism.
Product Information
Format
Purified
Presentation
Purified mouse monolconal IgG1κ in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05%NaN3
Applications
Application
Anti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P).
Key Applications
ELISA
Immunohistochemistry (Paraffin)
Application Notes
ELISA (Direct) Analysis:
Insulin-coated plate was incubated with Anti-Insulin, clone E11D7 in 2-fold serial dilutions diluted in PBST from 5 μg/mL to 0.01 μg/mL.
Biological Information
Immunogen
Human recombinant Insulin
Epitope
Insulin
Clone
E11D7
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes insulin
Isotype
IgG1κ
Species Reactivity
Human Rat Mouse
Species Reactivity Note
Human tested. Not tested with other species, but expected to react with Mouse and Rat based on sequence homology.
After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
FUNCTION:SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. SIZE:110 amino acids; 11981 Da SUBUNIT:Heterodimer of a B chain and an A chain linked by two disulfide bonds. SUBCELLULAR LOCATION:Secreted. DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730]. SIMILARITY:SwissProt: P01308 ## Belongs to the insulin family.
Product Usage Statements
Quality Assurance
Immunohistochemistry Analysis:
20 μg/mL of this antibody detected insulin in β cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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