ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion/migration. The kinase activity appears to be reduced following Integrin activation, and overexpression of p59ILK inhibits cellular adhesion to Integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).
Product Information
Format
Purified
Presentation
Protein G purified mouse monoclonal in storage buffer containing 0.1M Tris-Glycine, pH7.4, 15 mM NaCl, and 0.05% NaN3.
Applications
Application
Anti-ILK Antibody, clone 6G6.3 detects level of ILK & has been published & validated for use in WB.
Key Applications
Western Blotting
Application Notes
Not recommended for IP
Biological Information
Immunogen
Full length recombinant GST-tagged human ILK.
Clone
6G6.3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
FUNCTION:SwissProt: Q13418 # Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. SIZE:452 amino acids; 51419 Da SUBUNIT:Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts with LIMS1, LIMS2, parvins and probably TGFB1I1. SUBCELLULAR LOCATION:Cell junction, focal adhesion; Cytoplasmic side. TISSUE SPECIFICITY:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.DOMAIN:SwissProt: Q13418 A PH-like domain is involved in phosphatidylinositol phosphate binding. PTM:Autophosphorylated on serine residues. SIMILARITY:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 3 ANK repeats. & Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by western blot on HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
M, R, H
WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1051
Replaces
04-1149; MABT66
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-ILK Antibody, clone 6G6.3
Alternate Names
Integrin-Linked Kinase
p59ILK
DKFZp686F1765
p59
Background Information
ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion/migration. The kinase activity appears to be reduced following Integrin activation, and overexpression of p59ILK inhibits cellular adhesion to Integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).
Product Information
Format
Purified
Presentation
Protein G purified mouse monoclonal in storage buffer containing 0.1M Tris-Glycine, pH7.4, 15 mM NaCl, and 0.05% NaN3.
Applications
Application
Anti-ILK Antibody, clone 6G6.3 detects level of ILK & has been published & validated for use in WB.
Key Applications
Western Blotting
Application Notes
Not recommended for IP
Biological Information
Immunogen
Full length recombinant GST-tagged human ILK.
Clone
6G6.3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
FUNCTION:SwissProt: Q13418 # Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. SIZE:452 amino acids; 51419 Da SUBUNIT:Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts with LIMS1, LIMS2, parvins and probably TGFB1I1. SUBCELLULAR LOCATION:Cell junction, focal adhesion; Cytoplasmic side. TISSUE SPECIFICITY:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.DOMAIN:SwissProt: Q13418 A PH-like domain is involved in phosphatidylinositol phosphate binding. PTM:Autophosphorylated on serine residues. SIMILARITY:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 3 ANK repeats. & Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by western blot on HEK293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.
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