Ectonucleoside triphosphate diphosphohydrolase 1 (NTDase 1 or CD39) belongs to the membrane-bound ecto-nucleoside triphosphates diphosphohydrolase family (E-NTPDases) that are characterized for their ability to hydrolyze nucleoside di- and triphosphates, such as ATP, in the presence of cations, i.e. CA2+ and Mg2+. CD39 is an important membrane protein containing two transmembrane domains and a large NTPDase active, extracellular region containing apyrase conserved regions. It is heavily glycosolated, having six known N-glycosolation sites, and functions on the plasma membrane’s outer surface. CD39 initiates the metabolism, of ATP and ADP to adenosine through a 2-step exzymatic reaction, providing extracellular adenosine for the purpose of regulating various inflammatory endpoints. It is a putative marker for B-cell activation and its expression has also been observed on NK-cells, dendritic cells, T-cells, and vascular endothelial cells. CD39 is a known inhibitor of ADP-induced platelet aggregation and may also play a role in angiogenesis.
Product Information
Format
Purified
Control
BALB/c splenocytes
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
This Anti-CD39 Antibody, clone 5F2 is validated for use in FC, WB for the detection of CD39.
Key Applications
Flow Cytometry
Western Blotting
Application Notes
Western Blot Analysis: A previous lot was used by an independent laboratory in WB.
Biological Information
Immunogen
Recombinant protein corresponding to mouse CD39.
Epitope
Unknown
Clone
5F2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
SEQUENCE SIMILARITIES: Belongs to the GDA1/CD39 NTPase family.
Product Usage Statements
Quality Assurance
Evaluated by Flow Cytometry in BALB/c splenocytes.
Flow Cytometry Analysis: 0.1 µg/mL of this antibody detected CD39 in BALB/c splenocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
M
FC, WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-973
Description
Anti-CD39 Antibody, clone 5F2
Alternate Names
ectonucleoside triphosphate diphosphohydrolase 1
CD39 antigen
Ecto-apyrase
NTPDase 1
Lymphoid cell activation antigen
Ecto-ATP diphosphohydrolase
NTPDase-1
ecto-apyrase
ATPDase
Background Information
Ectonucleoside triphosphate diphosphohydrolase 1 (NTDase 1 or CD39) belongs to the membrane-bound ecto-nucleoside triphosphates diphosphohydrolase family (E-NTPDases) that are characterized for their ability to hydrolyze nucleoside di- and triphosphates, such as ATP, in the presence of cations, i.e. CA2+ and Mg2+. CD39 is an important membrane protein containing two transmembrane domains and a large NTPDase active, extracellular region containing apyrase conserved regions. It is heavily glycosolated, having six known N-glycosolation sites, and functions on the plasma membrane’s outer surface. CD39 initiates the metabolism, of ATP and ADP to adenosine through a 2-step exzymatic reaction, providing extracellular adenosine for the purpose of regulating various inflammatory endpoints. It is a putative marker for B-cell activation and its expression has also been observed on NK-cells, dendritic cells, T-cells, and vascular endothelial cells. CD39 is a known inhibitor of ADP-induced platelet aggregation and may also play a role in angiogenesis.
Product Information
Format
Purified
Control
BALB/c splenocytes
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
This Anti-CD39 Antibody, clone 5F2 is validated for use in FC, WB for the detection of CD39.
Key Applications
Flow Cytometry
Western Blotting
Application Notes
Western Blot Analysis: A previous lot was used by an independent laboratory in WB.
Biological Information
Immunogen
Recombinant protein corresponding to mouse CD39.
Epitope
Unknown
Clone
5F2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
SEQUENCE SIMILARITIES: Belongs to the GDA1/CD39 NTPase family.
Product Usage Statements
Quality Assurance
Evaluated by Flow Cytometry in BALB/c splenocytes.
Flow Cytometry Analysis: 0.1 µg/mL of this antibody detected CD39 in BALB/c splenocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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