Glycogen Synthase Kinase 3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
This Anti-GSK3α/β Antibody is validated for use in WB, IP for the detection of GSK3α/β.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation: 2-8 μL of a previous lot of antibody immunoprecipitated GSK3 alpha and beta from 100 μg of HEK293 RIPA lysate.
NOTE: This antibody also successfully immunoprecipitated GSK3 alpha and beta using the Catch and Release® v2.0 Reversible Immunoprecipitation System (Catalog # 17-500).
Biological Information
Immunogen
N-terminal 6His-tagged recombinant full length human GSK3β.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
GSK3 alpha and beta.
Isotype
IgG
Species Reactivity
Human Rat Mouse
Species Reactivity Note
Human and mouse.
Predicted to cross-react with rat based on sequence homology.
Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase,
homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of
several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription
factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)
FUNCTION:Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN By similarity. CATALYTIC ACTIVITY:ATP + [tau protein] = ADP + [tau protein] phosphate. SUBUNIT STRUCTURE:Monomer By similarity. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by western blot on lysates from HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GSK3 alpha and GSK3 beta in RIPA lysates from A431, HEK293, Jurkat, HeLa NE or 3T3/A31 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size
100 µl
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, R, M
WB, IP
Rb
Culture Supernatant
Monoclonal Antibody
Description
Catalogue Number
04-903
Replaces
05-903
Description
Anti-GSK3α/β Antibody, rabbit monoclonal
Alternate Names
GSK-3 alpha
glycogen synthase kinase 3 alpha
Background Information
Glycogen Synthase Kinase 3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
This Anti-GSK3α/β Antibody is validated for use in WB, IP for the detection of GSK3α/β.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation: 2-8 μL of a previous lot of antibody immunoprecipitated GSK3 alpha and beta from 100 μg of HEK293 RIPA lysate.
NOTE: This antibody also successfully immunoprecipitated GSK3 alpha and beta using the Catch and Release® v2.0 Reversible Immunoprecipitation System (Catalog # 17-500).
Biological Information
Immunogen
N-terminal 6His-tagged recombinant full length human GSK3β.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
GSK3 alpha and beta.
Isotype
IgG
Species Reactivity
Human Rat Mouse
Species Reactivity Note
Human and mouse.
Predicted to cross-react with rat based on sequence homology.
Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase,
homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of
several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription
factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)
FUNCTION:Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN By similarity. CATALYTIC ACTIVITY:ATP + [tau protein] = ADP + [tau protein] phosphate. SUBUNIT STRUCTURE:Monomer By similarity. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by western blot on lysates from HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GSK3 alpha and GSK3 beta in RIPA lysates from A431, HEK293, Jurkat, HeLa NE or 3T3/A31 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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