Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
Alternate Names
Proto-oncogene tyrosine-protein kinase Src
p60-Src
pp60c-src
Proto-oncogene c-Src
Background Information
Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
Product Information
Format
Culture Supernatant
Control
RIPA cell lysate
Presentation
Cultured supernantant in 0.05% sodium azide
Applications
Application
Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 is an antibody against phospho-Src family (Tyr416) for use in WB.
Key Applications
Western Blotting
Application Notes
Western Blot Analysis:
A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A).
Immunoprecipitation:
A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B).
Biological Information
Immunogen
KLH-conjugated, synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src
Clone
2N8
Host
Rabbit
Specificity
phospho-c-Src (Tyr416)
Isotype
IgG
Species Reactivity
Vertebrates Mouse
Species Reactivity Note
Mouse. Broad species cross-reactivity expected based on sequence homology.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
SIZE:536 amino acids; 59835 Da SUBUNIT:Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. PTM:Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY:SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot of endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186)
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
Vrt, M
WB
Rb
Culture Supernatant
Monoclonal Antibody
Description
Catalogue Number
04-857
Replaces
05-857
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
Alternate Names
Proto-oncogene tyrosine-protein kinase Src
p60-Src
pp60c-src
Proto-oncogene c-Src
Background Information
Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
Product Information
Format
Culture Supernatant
Control
RIPA cell lysate
Presentation
Cultured supernantant in 0.05% sodium azide
Applications
Application
Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 is an antibody against phospho-Src family (Tyr416) for use in WB.
Key Applications
Western Blotting
Application Notes
Western Blot Analysis:
A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A).
Immunoprecipitation:
A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B).
Biological Information
Immunogen
KLH-conjugated, synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src
Clone
2N8
Host
Rabbit
Specificity
phospho-c-Src (Tyr416)
Isotype
IgG
Species Reactivity
Vertebrates Mouse
Species Reactivity Note
Mouse. Broad species cross-reactivity expected based on sequence homology.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
SIZE:536 amino acids; 59835 Da SUBUNIT:Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. PTM:Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY:SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot of endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186)
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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