As members of the calcium-/calmodulin-depedent protein kinase superfamily, there are three known Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3. These serine/threonine kinases are involved in protein-protein and protein-carbohydrate adhesion and are also thought to be ion channel regulators. They are prominent downstream targets of protein kinase C and phospholipase D in various biological systems including mouse. PKDs are activated by various stimuli, including phorbol esters, G-protein-coupled receptors, reactive oxygen species, and diacylglycerol. PKDs regulate protein transport in the trans-Golgi network and are thought to play a role in muscle differentiation, cell motility, migration, and invasion. These isoforms are known to interact with each other. PKD2 is shown to be activated by phorbol esters, both in vivo and in vitro, and also by gastrin via the cholescystokinin/CCK(B) receptor in stably-transfected AGS-B cells. Furthermore, PKD2 is found to be active during the early phase of differentation of C2C12 cells into skeletal muscle cells. It is also reported to enhance TCR-induced NFAT activity in Jurkat T cells.
Product Information
Format
Purified
Control
RIPA lysates from HEK293 cells
Presentation
Cultured supernantant in 0.05% sodium azide
Applications
Application
Detect phospho-PKD (Ser916) using this Anti-phospho-PKD (Ser916) Antibody, clone MC29 validated for use in WB.
Key Applications
Western Blotting
Application Notes
Western Blot Analysis:
1:500-1:2000 of this lot detected PKD in RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate (PDBu) for 10 minutes at 50 ng/mL.
Biological Information
Immunogen
peptide containing RVpSIL in which pS corresponds to phospho-serine 916 of mouse PKD
Clone
MC29
Host
Rabbit
Specificity
phospho-PKD
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to cross-react with mouse based on sequence homology.
Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM]
routinely evaluated by immunoblot on RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
As members of the calcium-/calmodulin-depedent protein kinase superfamily, there are three known Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3. These serine/threonine kinases are involved in protein-protein and protein-carbohydrate adhesion and are also thought to be ion channel regulators. They are prominent downstream targets of protein kinase C and phospholipase D in various biological systems including mouse. PKDs are activated by various stimuli, including phorbol esters, G-protein-coupled receptors, reactive oxygen species, and diacylglycerol. PKDs regulate protein transport in the trans-Golgi network and are thought to play a role in muscle differentiation, cell motility, migration, and invasion. These isoforms are known to interact with each other. PKD2 is shown to be activated by phorbol esters, both in vivo and in vitro, and also by gastrin via the cholescystokinin/CCK(B) receptor in stably-transfected AGS-B cells. Furthermore, PKD2 is found to be active during the early phase of differentation of C2C12 cells into skeletal muscle cells. It is also reported to enhance TCR-induced NFAT activity in Jurkat T cells.
Product Information
Format
Purified
Control
RIPA lysates from HEK293 cells
Presentation
Cultured supernantant in 0.05% sodium azide
Applications
Application
Detect phospho-PKD (Ser916) using this Anti-phospho-PKD (Ser916) Antibody, clone MC29 validated for use in WB.
Key Applications
Western Blotting
Application Notes
Western Blot Analysis:
1:500-1:2000 of this lot detected PKD in RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate (PDBu) for 10 minutes at 50 ng/mL.
Biological Information
Immunogen
peptide containing RVpSIL in which pS corresponds to phospho-serine 916 of mouse PKD
Clone
MC29
Host
Rabbit
Specificity
phospho-PKD
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to cross-react with mouse based on sequence homology.
Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM]
routinely evaluated by immunoblot on RIPA lysates from HEK293 cells stimulated with phorbol 12,13-dibutyrate
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
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