TNF receptor-associated factor 6 is a member of the TNF receptor associated factor (TRAF) protein family. TNF receptor-associated factor 6 proteins play an important role as adaptor protein for cell survival, proliferation, differentiation and stress responses. TNF receptor-associated factor 6 has unique binding behavior that result in signaling responses from the interleukin-1 receptor (IL-1R) toll-like receptor. TNF receptor-associated factor 6 mediates signaling indirectly with IL-1R/TLR through IRAK. This leads to activation of NF-κB and MAP kinase signaling pathways though downstream association with the TAB/TAK-1 complex. TNF receptor-associated factor 6 is also involved in the activation of Akt/PKB and serves as a direct E3 ligase which is essential for Akt ubiquitination.
Product Information
Format
Purified
Presentation
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-TRAF6 Antibody, Rabbit detects level of TRAF6 & has been published & validated for use in WB, IH(P).
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Biological Information
Immunogen
KLH-conjugated synthetic peptide composed of residues at the N-terminus of human TRAF6.
Host
Rabbit
Specificity
Recognizes a region composed of residues at the N-terminus of human TRAF6.
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported.
FUNCTION:SwissProt: Q9Y4K3 # Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Also involved in the IL-1 signaling pathway via MYD88 and IRAK kinases. Seems to be involved in IL-17 signaling (By similarity). Mediates activation of NF-kappa-B and JNK. May function as an E3 ubiquitin ligase. SIZE:522 amino acids; 59573 Da SUBUNIT:Homotrimer (Probable). Binds to TNFRSF5/CD40 and TNFRSF11A/RANK. Associates with NGFR, TNFRSF17, IRAK1, IRAK2, IRAK3, IRAK4, RIPK2, MAP3K1, MAP3K5, MAP3K14, CSK, and TRAF- interacting protein TRIP and TRAF and TNF receptor associated protein TTRAP. Interacts with IL17R. Interacts with SQSTM1 bridging NTRK1 and NGFR. Forms a ternary complex with SQSTM1 and PRKCZ (By similarity). Interacts with PELI1, PELI2 and PELI3. Binds UBE2V1. Interacts with MAVS/IPS1. Interacts with TAX1BP1. Interacts with IL1RL1. Interacts with TRAFD1. Interacts with ZNF675. SUBCELLULAR LOCATION:Cytoplasm (Probable).TISSUE SPECIFICITY: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.DOMAIN:SwissProt: Q9Y4K3 The coiled coil domain mediates homo- and hetero- oligomerization. & The MATH/TRAF domain binds to receptor cytoplasmic domains. PTM:Polyubiquitinated (By similarity). SIMILARITY:Contains 1 MATH domain. & Contains 1 RING-type zinc finger. & Contains 2 TRAF-type zinc fingers.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
R, H, M
IH(P), WB
Rb
Purified
Monoclonal Antibody
Description
Catalogue Number
04-451
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-TRAF6 Antibody, rabbit monoclonal
Background Information
TNF receptor-associated factor 6 is a member of the TNF receptor associated factor (TRAF) protein family. TNF receptor-associated factor 6 proteins play an important role as adaptor protein for cell survival, proliferation, differentiation and stress responses. TNF receptor-associated factor 6 has unique binding behavior that result in signaling responses from the interleukin-1 receptor (IL-1R) toll-like receptor. TNF receptor-associated factor 6 mediates signaling indirectly with IL-1R/TLR through IRAK. This leads to activation of NF-κB and MAP kinase signaling pathways though downstream association with the TAB/TAK-1 complex. TNF receptor-associated factor 6 is also involved in the activation of Akt/PKB and serves as a direct E3 ligase which is essential for Akt ubiquitination.
Product Information
Format
Purified
Presentation
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-TRAF6 Antibody, Rabbit detects level of TRAF6 & has been published & validated for use in WB, IH(P).
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Biological Information
Immunogen
KLH-conjugated synthetic peptide composed of residues at the N-terminus of human TRAF6.
Host
Rabbit
Specificity
Recognizes a region composed of residues at the N-terminus of human TRAF6.
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported.
FUNCTION:SwissProt: Q9Y4K3 # Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Also involved in the IL-1 signaling pathway via MYD88 and IRAK kinases. Seems to be involved in IL-17 signaling (By similarity). Mediates activation of NF-kappa-B and JNK. May function as an E3 ubiquitin ligase. SIZE:522 amino acids; 59573 Da SUBUNIT:Homotrimer (Probable). Binds to TNFRSF5/CD40 and TNFRSF11A/RANK. Associates with NGFR, TNFRSF17, IRAK1, IRAK2, IRAK3, IRAK4, RIPK2, MAP3K1, MAP3K5, MAP3K14, CSK, and TRAF- interacting protein TRIP and TRAF and TNF receptor associated protein TTRAP. Interacts with IL17R. Interacts with SQSTM1 bridging NTRK1 and NGFR. Forms a ternary complex with SQSTM1 and PRKCZ (By similarity). Interacts with PELI1, PELI2 and PELI3. Binds UBE2V1. Interacts with MAVS/IPS1. Interacts with TAX1BP1. Interacts with IL1RL1. Interacts with TRAFD1. Interacts with ZNF675. SUBCELLULAR LOCATION:Cytoplasm (Probable).TISSUE SPECIFICITY: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.DOMAIN:SwissProt: Q9Y4K3 The coiled coil domain mediates homo- and hetero- oligomerization. & The MATH/TRAF domain binds to receptor cytoplasmic domains. PTM:Polyubiquitinated (By similarity). SIMILARITY:Contains 1 MATH domain. & Contains 1 RING-type zinc finger. & Contains 2 TRAF-type zinc fingers.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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