In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Caspase-9 (Pro) using this Anti-Caspase-9 (Pro) Antibody, Rabbit validated for use in FC, WB, IH(P).
Key Applications
Immunohistochemistry (Paraffin)
Flow Cytometry
Western Blotting
Biological Information
Immunogen
KLH-conjugated synthetic peptide composed of residues at the N-terminus of human Caspase-9.
Host
Rabbit
Specificity
Recognizes a region composed of residues at the N-terminus of human Pro-Caspase-9. This antibody only binds to the pro-form of Caspase-9.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
FUNCTION:SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. SIZE:416 amino acids; 46281 Da SUBUNIT:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7. TISSUE SPECIFICITY:Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. PTM:Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase- 3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. SIMILARITY:SwissProt: P55211 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
R, H, M
IH(P), FC, WB
Rb
Purified
Monoclonal Antibody
Description
Catalogue Number
04-443
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-Caspase-9 (Pro) Antibody, rabbit monoclonal
Product Information
Format
Purified
Presentation
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Caspase-9 (Pro) using this Anti-Caspase-9 (Pro) Antibody, Rabbit validated for use in FC, WB, IH(P).
Key Applications
Immunohistochemistry (Paraffin)
Flow Cytometry
Western Blotting
Biological Information
Immunogen
KLH-conjugated synthetic peptide composed of residues at the N-terminus of human Caspase-9.
Host
Rabbit
Specificity
Recognizes a region composed of residues at the N-terminus of human Pro-Caspase-9. This antibody only binds to the pro-form of Caspase-9.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
FUNCTION:SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. SIZE:416 amino acids; 46281 Da SUBUNIT:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7. TISSUE SPECIFICITY:Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. PTM:Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase- 3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. SIMILARITY:SwissProt: P55211 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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