PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
Product Information
Format
Purified
Control
Jurkat cell lysate
Presentation
Purified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p110γ validated for use in FC, IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunoprecipitation
Application Notes
Immunoprecipitation:
A 1:50 dilution of a previous lot of antibody immunoprecipitated PI3K, p110γ using protein G:agarose beads.
Flow Cytometry:
A 1:50 of a previous lot of this antibody stained cells for intracellular staining for flow Cytometry analysis.
Biological Information
Immunogen
KLH-conjugated synthetic peptide corresponding to the N-terminus human PI3 Kinase, p110γ
Epitope
N-terminus
Host
Rabbit
Specificity
This antibody recognizes the N-terminus human PI3 Kinase, p110γ.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Proven to react with human. Reactivity with other species has not been confirmed.
This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.
FUNCTION: SwissProt: P48736 # 3-phosphorylates the cellular phosphoinositide PtdIns- 4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5- triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
SIZE: 1102 amino acids; 126454 Da
SUBUNIT: Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit.
TISSUE SPECIFICITY: Pancreas, skeletal muscle, liver and heart.
SIMILARITY: SwissProt: P48736 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on jurkat cell lysate.
Western Blot Analysis:
A 1:1,000 dilution of the lot detected endogenous PI3 Kinase, p110γ in lysates from Jurkat cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
Product Information
Format
Purified
Control
Jurkat cell lysate
Presentation
Purified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p110γ validated for use in FC, IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunoprecipitation
Application Notes
Immunoprecipitation:
A 1:50 dilution of a previous lot of antibody immunoprecipitated PI3K, p110γ using protein G:agarose beads.
Flow Cytometry:
A 1:50 of a previous lot of this antibody stained cells for intracellular staining for flow Cytometry analysis.
Biological Information
Immunogen
KLH-conjugated synthetic peptide corresponding to the N-terminus human PI3 Kinase, p110γ
Epitope
N-terminus
Host
Rabbit
Specificity
This antibody recognizes the N-terminus human PI3 Kinase, p110γ.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Proven to react with human. Reactivity with other species has not been confirmed.
This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.
FUNCTION: SwissProt: P48736 # 3-phosphorylates the cellular phosphoinositide PtdIns- 4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5- triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
SIZE: 1102 amino acids; 126454 Da
SUBUNIT: Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit.
TISSUE SPECIFICITY: Pancreas, skeletal muscle, liver and heart.
SIMILARITY: SwissProt: P48736 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on jurkat cell lysate.
Western Blot Analysis:
A 1:1,000 dilution of the lot detected endogenous PI3 Kinase, p110γ in lysates from Jurkat cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
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