NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumor promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK, TRAF-2 and TRIF (4).
Product Information
Format
Unpurified
Control
HeLa cell lysate
Presentation
Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide.
Applications
Application
Anti-TBK1/NAK (N-term) Antibody, clone EP611Y, Rabbit Monoclonal is an antibody against TBK1/NAK (N-term) for use in Western Blotting, ICC, Immunoprecipitation, IHC(P).
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot was used in human breast carcinoma tissue.
Immunocytochemistry Analysis: A 1:250-500 dilution from a representative lot was used in IC.
Immunoprecipitation Analysis: A 1:10-100 dilution from a representative lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to the N-terminus of human TBK1/NAK.
Epitope
N-terminus
Clone
EP611Y
Host
Rabbit
Specificity
This antibody recognizes the N-terminus of TBK1/NAK.
Evaluated by Western Blot in HeLa cell lysate
WesternBlot Analysis: A 1:1,000 dilution of this antibody detected TBK1/NAK in HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumor promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK, TRAF-2 and TRIF (4).
Product Information
Format
Unpurified
Control
HeLa cell lysate
Presentation
Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide.
Applications
Application
Anti-TBK1/NAK (N-term) Antibody, clone EP611Y, Rabbit Monoclonal is an antibody against TBK1/NAK (N-term) for use in Western Blotting, ICC, Immunoprecipitation, IHC(P).
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot was used in human breast carcinoma tissue.
Immunocytochemistry Analysis: A 1:250-500 dilution from a representative lot was used in IC.
Immunoprecipitation Analysis: A 1:10-100 dilution from a representative lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to the N-terminus of human TBK1/NAK.
Epitope
N-terminus
Clone
EP611Y
Host
Rabbit
Specificity
This antibody recognizes the N-terminus of TBK1/NAK.
Evaluated by Western Blot in HeLa cell lysate
WesternBlot Analysis: A 1:1,000 dilution of this antibody detected TBK1/NAK in HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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