Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Applications
Application
Anti-MKK4 CT Antibody is an antibody against MKK4 CT for use in FC, IH(P), IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Western Blot: 1:1000.
Immunoprecipitation: 1:50. The use of protein G agarose is recommended.
Flow Cytometry: 1:50.
Immunohistochemistry: 1:50-1:100 on paraffin-embeddedsections of human lung adenocarcinoma.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
KLH-conjugated synthetic peptide corresponding to residues in the C-term of human MKK4.
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
FUNCTION:SwissProt: P45985 # Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). SIZE:399 amino acids; 44288 Da SUBUNIT:Interacts with SPAG9 (By similarity). TISSUE SPECIFICITY:Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. PTM:Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases (By similarity). SIMILARITY:SwissProt: P45985 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot analysis.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
2 years at -20°C from date of shipment.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
R, H, M
WB, FC, IH(P), IP
Rb
Purified
Monoclonal Antibody
Description
Catalogue Number
04-382
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-MKK4 CT Antibody, rabbit monoclonal
Alternate Names
SERK1
JNKK
JNKK1
MAPKK4
MEK4
MKK4
PRKMK4
SEK1
Product Information
Format
Purified
Presentation
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Applications
Application
Anti-MKK4 CT Antibody is an antibody against MKK4 CT for use in FC, IH(P), IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Western Blot: 1:1000.
Immunoprecipitation: 1:50. The use of protein G agarose is recommended.
Flow Cytometry: 1:50.
Immunohistochemistry: 1:50-1:100 on paraffin-embeddedsections of human lung adenocarcinoma.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
KLH-conjugated synthetic peptide corresponding to residues in the C-term of human MKK4.
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
FUNCTION:SwissProt: P45985 # Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). SIZE:399 amino acids; 44288 Da SUBUNIT:Interacts with SPAG9 (By similarity). TISSUE SPECIFICITY:Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. PTM:Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases (By similarity). SIMILARITY:SwissProt: P45985 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot analysis.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
