Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-MKK3 NT Antibody detects level of MKK3 NT & has been published & validated for use in FC, IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunoprecipitation
Application Notes
Western Blot: 1,5,000-1:20,000.
Immunoprecipitation: 1:50. The use of protein G agarose is recommended.
Flow Cytometry: 1:50.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
KLH-conjugated synthetic peptide corresponding to residues in the N-term of human MKK3.
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
FUNCTION:SwissProt: P46734 # Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. SIZE:347 amino acids; 39318 Da SUBUNIT:Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2. Interacts with Yersinia yopJ. TISSUE SPECIFICITY:Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. PTM:Autophosphorylated. & Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. DISEASE:SwissProt: P46734 # Defects in MAP2K3 may be involved in colon cancer. SIMILARITY:SwissProt: P46734 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot analysis.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
2 years at -20°C from date of shipment.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
WB, FC, IP
Rb
Purified
Monoclonal Antibody
Description
Catalogue Number
04-381
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-MKK3 NT Antibody, rabbit monoclonal
Alternate Names
PRKMK3
MAPKK3
MEK3
Product Information
Format
Purified
Control
POSITIVE: Jurkat, HeLa, MCF-7 or K562 cells.
Presentation
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-MKK3 NT Antibody detects level of MKK3 NT & has been published & validated for use in FC, IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunoprecipitation
Application Notes
Western Blot: 1,5,000-1:20,000.
Immunoprecipitation: 1:50. The use of protein G agarose is recommended.
Flow Cytometry: 1:50.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
KLH-conjugated synthetic peptide corresponding to residues in the N-term of human MKK3.
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
FUNCTION:SwissProt: P46734 # Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. SIZE:347 amino acids; 39318 Da SUBUNIT:Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2. Interacts with Yersinia yopJ. TISSUE SPECIFICITY:Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. PTM:Autophosphorylated. & Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. DISEASE:SwissProt: P46734 # Defects in MAP2K3 may be involved in colon cancer. SIMILARITY:SwissProt: P46734 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot analysis.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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