The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
FUNCTION:SwissProt: Q9Y478 # AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. Also regulates cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase and hormone- sensitive lipase. This is a regulatory subunit, may be a positive regulator of AMPK activity. It may also serve as an adaptor molecule for the catalytic alpha-subunit. SIZE:270 amino acids; 30382 Da SUBUNIT:Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic regulatory subunits. Interacts with FNIP1. PTM:Phosphorylated (By similarity). SIMILARITY:SwissProt: Q9Y478 ## Belongs to the 5'-AMP-activated protein kinase beta subunit family.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
R, H, M
WB, FC, IH(P), IP
Rb
Purified
Monoclonal Antibody
Description
Catalogue Number
04-324
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-AMPKβ1 Antibody, rabbit monoclonal
Alternate Names
protein kinase, AMP-activated, beta 1 non-catalytic subunit
PRKAB1
AMPK
AMPKb1
AMPK beta 1
protein kinase
AMP-activated
Product Information
Format
Purified
Presentation
In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Anti-AMPKβ1 Antibody, Rabbit detects level of AMPKβ1 & has been published & validated for use in FC, IH(P), IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Biological Information
Immunogen
KLH-conjugated synthetic peptide corresponding to human AMPKβ1.
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
FUNCTION:SwissProt: Q9Y478 # AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. Also regulates cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase and hormone- sensitive lipase. This is a regulatory subunit, may be a positive regulator of AMPK activity. It may also serve as an adaptor molecule for the catalytic alpha-subunit. SIZE:270 amino acids; 30382 Da SUBUNIT:Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic regulatory subunits. Interacts with FNIP1. PTM:Phosphorylated (By similarity). SIMILARITY:SwissProt: Q9Y478 ## Belongs to the 5'-AMP-activated protein kinase beta subunit family.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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