Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.
Product Information
Format
Culture Supernatant
Control
HeLa whole cell lysate.
Presentation
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin B1 (C-term) Antibody, clone Y106, rabbit is an antibody against Cyclin B1 (C-term) for use in FC, IC, IH, IH(P), IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunohistochemistry: 1:100 dilution from a previous lot was used on immunohistochemistry.
Immunocytochemistry: 1:50 dilution from a previous lot was used on immunocytochemistry.
Immunoprecipitation: 1:100 dilution from a previous lot was used on immunoprecipitation.
Flow Cytometry: 1:100 dilution from a previous lot was used on flow cytometry.
Biological Information
Immunogen
Synthetic peptide identical to the C-terminus of human Cyclin B1.
Epitope
C-terminus
Clone
Y106
Host
Rabbit
Specificity
This antibody recognizes Cyclin B1 at the C-Terminus.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Proven to react with human. Not expected to react with mouse and rat.
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]
Function:Essential for the control of the cell cycle at the G2/M (mitosis) transition. Ref.10 Ref.11 Subunit structure:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Ref.1 Ref.5 Ref.6 Subcellular location:Cytoplasm. Nucleus. Centrosome. Ref.8 Developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis. Ref.1 Post-translational modification:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Ref.7 Sequence similarities:Belongs to the cyclin family. Cyclin AB subfamily.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on HeLa whole cell lysate.
Western Blot Analysis:
A 1:5,000-1:10,000 dilution of this lot was used to detect Cyclin B1 on HeLa whole cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.
Product Information
Format
Culture Supernatant
Control
HeLa whole cell lysate.
Presentation
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin B1 (C-term) Antibody, clone Y106, rabbit is an antibody against Cyclin B1 (C-term) for use in FC, IC, IH, IH(P), IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunohistochemistry: 1:100 dilution from a previous lot was used on immunohistochemistry.
Immunocytochemistry: 1:50 dilution from a previous lot was used on immunocytochemistry.
Immunoprecipitation: 1:100 dilution from a previous lot was used on immunoprecipitation.
Flow Cytometry: 1:100 dilution from a previous lot was used on flow cytometry.
Biological Information
Immunogen
Synthetic peptide identical to the C-terminus of human Cyclin B1.
Epitope
C-terminus
Clone
Y106
Host
Rabbit
Specificity
This antibody recognizes Cyclin B1 at the C-Terminus.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Proven to react with human. Not expected to react with mouse and rat.
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]
Function:Essential for the control of the cell cycle at the G2/M (mitosis) transition. Ref.10 Ref.11 Subunit structure:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Ref.1 Ref.5 Ref.6 Subcellular location:Cytoplasm. Nucleus. Centrosome. Ref.8 Developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis. Ref.1 Post-translational modification:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Ref.7 Sequence similarities:Belongs to the cyclin family. Cyclin AB subfamily.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on HeLa whole cell lysate.
Western Blot Analysis:
A 1:5,000-1:10,000 dilution of this lot was used to detect Cyclin B1 on HeLa whole cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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