CREB, cyclic AMP (cAMP) response element-binding protein, is a ubiquitously expressed basic leucine zipper transcription factor that functions in glucose homeostasis and stimulates the expression of numerous genes in response to growth factors, hormones, neurotransmitters, ion fluxes, stress signals, and has been implicated in memory because if its apparent role in synaptic plasticity. CREB functions either by itself or with related family members, which include CREB, CREM, and ATF-1, by forming homo- and heterodimers through their basic leucine zipper (bZIP) domains. The CREB signaling pathway is activated by extracellular ligands that bind to cell surface receptors and relay their message through various intracellular second messengers resulting ultimately in the activation of nuclear CREB through phosphorylation of the transactivation domain at serine 1332,4. The classical model of CREB activation is through PKA (cAMP-dependent protein kinase). In the non-active state, PKA resides in the cytoplasm as an inactive heterotetramer of paired regulatory and catalytic subunits. Stimulation of cAMP causes the releases of the catalytic subunits of PKA that passively diffuse into the nucleus and initiates gene expression by phosphorylating CREB at its transactivation domain on serine 1331,2,3,4. CREB mediates the activation of cAMP-responsive genes by binding as a dimer to a conserved cAMP-responsive element (CRE), 5’-TGACGTCA-3’, located within the promoter region of target genes. Target gene activation is terminated by the serine/threonine phosphatases PP-1-mediated dephospho-rylation of CREB.
Product Information
Format
Culture Supernatant
Control
A431 whole cell lysate.
Presentation
100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-CREB Antibody, clone E306, rabbit detects level of CREB & has been published & validated for use in FC, IC, IF, IH(P), IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Flow Cytometry: 1:40 dilution from a previous lot was used on flow cytometry.
Immunohistochemistry: 1:250-500 dilution from a previous lot was used on immunohistochemistry.
Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation.
Immunocytochemistry: 1:250-500 dilution from a previous lot was used on immunocytochemistry.
Function:This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. Subunit structure:Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP By similarity. Subcellular location:Nucleus. Post-translational modification:Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. Sequence similarities:Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on A431 whole cell lysate.
Western Blot Analysis:
A 1:1000 dilution of this lot was used to detect CREB in A431 whole cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
R, H, M
WB, FC, ICC, IF, IH(P), IP
Rb
Culture Supernatant
Monoclonal Antibody
Description
Catalogue Number
04-218
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-CREB Antibody, clone E306, rabbit monoclonal
Alternate Names
transactivator protein
cAMP responsive element binding protein
active transcription factor CREB
Background Information
CREB, cyclic AMP (cAMP) response element-binding protein, is a ubiquitously expressed basic leucine zipper transcription factor that functions in glucose homeostasis and stimulates the expression of numerous genes in response to growth factors, hormones, neurotransmitters, ion fluxes, stress signals, and has been implicated in memory because if its apparent role in synaptic plasticity. CREB functions either by itself or with related family members, which include CREB, CREM, and ATF-1, by forming homo- and heterodimers through their basic leucine zipper (bZIP) domains. The CREB signaling pathway is activated by extracellular ligands that bind to cell surface receptors and relay their message through various intracellular second messengers resulting ultimately in the activation of nuclear CREB through phosphorylation of the transactivation domain at serine 1332,4. The classical model of CREB activation is through PKA (cAMP-dependent protein kinase). In the non-active state, PKA resides in the cytoplasm as an inactive heterotetramer of paired regulatory and catalytic subunits. Stimulation of cAMP causes the releases of the catalytic subunits of PKA that passively diffuse into the nucleus and initiates gene expression by phosphorylating CREB at its transactivation domain on serine 1331,2,3,4. CREB mediates the activation of cAMP-responsive genes by binding as a dimer to a conserved cAMP-responsive element (CRE), 5’-TGACGTCA-3’, located within the promoter region of target genes. Target gene activation is terminated by the serine/threonine phosphatases PP-1-mediated dephospho-rylation of CREB.
Product Information
Format
Culture Supernatant
Control
A431 whole cell lysate.
Presentation
100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-CREB Antibody, clone E306, rabbit detects level of CREB & has been published & validated for use in FC, IC, IF, IH(P), IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Flow Cytometry: 1:40 dilution from a previous lot was used on flow cytometry.
Immunohistochemistry: 1:250-500 dilution from a previous lot was used on immunohistochemistry.
Immunoprecipitation: 1:80 dilution from a previous lot was used on immunoprecipitation.
Immunocytochemistry: 1:250-500 dilution from a previous lot was used on immunocytochemistry.
Function:This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. Subunit structure:Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP By similarity. Subcellular location:Nucleus. Post-translational modification:Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. Sequence similarities:Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on A431 whole cell lysate.
Western Blot Analysis:
A 1:1000 dilution of this lot was used to detect CREB in A431 whole cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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