Cdc25A, B and C are dual-specificity phosphatases that activate cyclin-dependent kinases by removing inhibitory phosphates. Their activity is timed at specific points in the cell cycle, to ensure appropriate cell cycle progression, and the checkpoint kinases activated by DNA damage or other replication blocks, phosphorylate and inhibit the cdc25 proteins.
Product Information
Format
Culture Supernatant
Control
Untreated and Calyculin/Okadaic acid-treated A431 or HeLa Cell lysate.
Presentation
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA
Applications
Application
This Anti-phospho-Cdc25C (Ser216) Antibody, clone E190, Rabbit is validated for use in FC, IC, IH(P), IP, WB for the detection of phospho-Cdc25C (Ser216).
Key Applications
Western Blotting
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemisty.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Biological Information
Immunogen
Synthetic peptide directed against the region encompassing human phosphorylated Cdc25C on Serine 216.
Epitope
Ser216
Clone
E190
Host
Rabbit
Specificity
This antibody recognizes phosphorylated Cdc25c at ser216.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Proven to react with human. Not expected to react with rat and mouse.
This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded
protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq].
FUNCTION:Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDC2 and activate its kinase activity.
STRUCTURE:Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity. Ref.8
LOCATION:Nucleus.
Developmental stage Expressed predominantly in G2 phase.
PTM:Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Ref.7 Ref.9 Ref.10 Ref.11
SIMILARITY:Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on untreated and Calyculin/Okadaic acid-treated A431 cell lysate.
Western Blot Analysis: A 1:250-1:1,000 dilution of this lot was used to detect phosphorylated Cdc25C (Ser216) in untreated and Calyculin/Okadaic acid-treated A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Cdc25A, B and C are dual-specificity phosphatases that activate cyclin-dependent kinases by removing inhibitory phosphates. Their activity is timed at specific points in the cell cycle, to ensure appropriate cell cycle progression, and the checkpoint kinases activated by DNA damage or other replication blocks, phosphorylate and inhibit the cdc25 proteins.
Product Information
Format
Culture Supernatant
Control
Untreated and Calyculin/Okadaic acid-treated A431 or HeLa Cell lysate.
Presentation
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA
Applications
Application
This Anti-phospho-Cdc25C (Ser216) Antibody, clone E190, Rabbit is validated for use in FC, IC, IH(P), IP, WB for the detection of phospho-Cdc25C (Ser216).
Key Applications
Western Blotting
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemisty.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Biological Information
Immunogen
Synthetic peptide directed against the region encompassing human phosphorylated Cdc25C on Serine 216.
Epitope
Ser216
Clone
E190
Host
Rabbit
Specificity
This antibody recognizes phosphorylated Cdc25c at ser216.
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Proven to react with human. Not expected to react with rat and mouse.
This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded
protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq].
FUNCTION:Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDC2 and activate its kinase activity.
STRUCTURE:Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity. Ref.8
LOCATION:Nucleus.
Developmental stage Expressed predominantly in G2 phase.
PTM:Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Ref.7 Ref.9 Ref.10 Ref.11
SIMILARITY:Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on untreated and Calyculin/Okadaic acid-treated A431 cell lysate.
Western Blot Analysis: A 1:250-1:1,000 dilution of this lot was used to detect phosphorylated Cdc25C (Ser216) in untreated and Calyculin/Okadaic acid-treated A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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