Interleukin 21 (IL-21) is a cytokine that has immunoregulatory activity on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. IL-21 induces cell division/proliferation in its target cells and is expressed in activated human CD4+ T cells. IL-21 may promote the transition between innate and adaptive immunity and may play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. IL-21 expression is up-regulated in Th2 and Th17 subsets of T helper cells, as well as follicular T cells. Furthermore IL-21 is expressed in NK T cells regulating the function of these cells. IL-21 is also produced by Hodgkin's lymphoma (HL) cancer cells and may explain a great deal of the behavior of classical Hodgkin's lymphoma including clusters of other immune cells gathered around HL cells in cultures.
Product Information
Format
Purified
Control
Human IL-21 recombinant protein
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
This Anti-Interleukin 21 (IL-21) Antibody, clone 1G8 is validated for use in WB, ELISA for the detection of Interleukin 21 (IL-21).
Key Applications
ELISA
Western Blotting
Application Notes
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Dr. Gerard Zurawski, Baylor Institute for Immunology Research, Dallas, TX)
Biological Information
Immunogen
Full length recombinant protein corresponding to human IL-21.
Clone
AB43.50.32H5.1G8
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG1 and IgG3 in B-cells By similarity. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
SUBCELLULAR LOCATION: Secreted
TISSUE SPECIFICITY: Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
SEQUENCE SIMLARITIES: Belongs to the IL-15/IL-21 family.
SEQUENCE CAUTION: The sequence AAG29348.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66258.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66259.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66260.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66261.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66262.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH69124.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAU88182.1 differs from that shown. Reason: Erroneous initiation.
The sequence ABG36529.1 differs from that shown. Reason: Erroneous initiation.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot from human IL-21 recombinant protein.
Western Blot Analysis: 0.5 µg/mL of this antibody detected IL-21 on 10 µg of human IL-21 recombinant protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
ELISA, WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-1583
Description
Anti-Interleukin 21 (IL-21) Antibody, clone 1G8
Alternate Names
interleukin 2
interleukin-2
interleukin-21 isoform
Background Information
Interleukin 21 (IL-21) is a cytokine that has immunoregulatory activity on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. IL-21 induces cell division/proliferation in its target cells and is expressed in activated human CD4+ T cells. IL-21 may promote the transition between innate and adaptive immunity and may play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. IL-21 expression is up-regulated in Th2 and Th17 subsets of T helper cells, as well as follicular T cells. Furthermore IL-21 is expressed in NK T cells regulating the function of these cells. IL-21 is also produced by Hodgkin's lymphoma (HL) cancer cells and may explain a great deal of the behavior of classical Hodgkin's lymphoma including clusters of other immune cells gathered around HL cells in cultures.
Product Information
Format
Purified
Control
Human IL-21 recombinant protein
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
This Anti-Interleukin 21 (IL-21) Antibody, clone 1G8 is validated for use in WB, ELISA for the detection of Interleukin 21 (IL-21).
Key Applications
ELISA
Western Blotting
Application Notes
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Dr. Gerard Zurawski, Baylor Institute for Immunology Research, Dallas, TX)
Biological Information
Immunogen
Full length recombinant protein corresponding to human IL-21.
Clone
AB43.50.32H5.1G8
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG1 and IgG3 in B-cells By similarity. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
SUBCELLULAR LOCATION: Secreted
TISSUE SPECIFICITY: Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
SEQUENCE SIMLARITIES: Belongs to the IL-15/IL-21 family.
SEQUENCE CAUTION: The sequence AAG29348.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66258.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66259.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66260.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66261.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH66262.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH69124.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAU88182.1 differs from that shown. Reason: Erroneous initiation.
The sequence ABG36529.1 differs from that shown. Reason: Erroneous initiation.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot from human IL-21 recombinant protein.
Western Blot Analysis: 0.5 µg/mL of this antibody detected IL-21 on 10 µg of human IL-21 recombinant protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
