Interleukin-13 (IL-13) is a cytokine that is secreted by helper T cells in reaction to T cell antigen receptor signaling. IL-13 is also secreted by mast cells and basophils when cross-linking with immunoglobulin E. IL-13 seems to play a role as an effector in ulcerative colitis that weakens epithelial barrier function by influencing epithelial apoptosis, tight junctions and restitution velocity. IL-13 has been shown to be involved in the pathogenesis of allergic diseases such as atopic dermatitis. IL-13 seems to also have a role to play in alcohol mediated activation of pro-fibrotic pathways. IL-13 is thought to be an important cytokine during the expansion of asthma and chronic obstructive pulmonary disease and may be critical clinical therapy targets.
Product Information
Format
Purified
Control
Human IL-13 recombinant protein
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Detect Interleukin-13 (IL-13) using this Anti-Interleukin-13 (IL-13) Antibody, clone B2 validated for use in WB.
Key Applications
Western Blotting
Application Notes
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA (Dr. Gerard Zurawski, Baylor Institute for Immunology Research, Dallas, TX).
Biological Information
Immunogen
Full-length recombinant protein corresponding to human IL-13.
Clone
AB31.56.13G1.B2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq].
FUNCTION: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.
SUBCELLULAR LOCATION: Secreted.
INVOLVEMENT IN DISEASE: Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH) [MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.
SEQUENCE SIMILARITIES: Belongs to the IL-4/IL-13 family.
CAUTION: It is uncertain whether Met-1 or Met-15 is the initiator.
SEQUENCE CAUTION: The sequence AAA36107.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAA83738.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAB01681.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAC03535.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAK53823.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence CAA48824.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in lysates from cells expressing human IL-13 recombinant protein.
Western Blot Analysis: 0.5 µg/mL of this antibody detected 10 µg of human IL-13 recombinant protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-1579
Description
Anti-Interleukin-13 (IL-13) Antibody, clone B2
Alternate Names
interleukin-13
interleukin 13
Background Information
Interleukin-13 (IL-13) is a cytokine that is secreted by helper T cells in reaction to T cell antigen receptor signaling. IL-13 is also secreted by mast cells and basophils when cross-linking with immunoglobulin E. IL-13 seems to play a role as an effector in ulcerative colitis that weakens epithelial barrier function by influencing epithelial apoptosis, tight junctions and restitution velocity. IL-13 has been shown to be involved in the pathogenesis of allergic diseases such as atopic dermatitis. IL-13 seems to also have a role to play in alcohol mediated activation of pro-fibrotic pathways. IL-13 is thought to be an important cytokine during the expansion of asthma and chronic obstructive pulmonary disease and may be critical clinical therapy targets.
Product Information
Format
Purified
Control
Human IL-13 recombinant protein
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Detect Interleukin-13 (IL-13) using this Anti-Interleukin-13 (IL-13) Antibody, clone B2 validated for use in WB.
Key Applications
Western Blotting
Application Notes
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA (Dr. Gerard Zurawski, Baylor Institute for Immunology Research, Dallas, TX).
Biological Information
Immunogen
Full-length recombinant protein corresponding to human IL-13.
Clone
AB31.56.13G1.B2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq].
FUNCTION: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.
SUBCELLULAR LOCATION: Secreted.
INVOLVEMENT IN DISEASE: Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH) [MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.
SEQUENCE SIMILARITIES: Belongs to the IL-4/IL-13 family.
CAUTION: It is uncertain whether Met-1 or Met-15 is the initiator.
SEQUENCE CAUTION: The sequence AAA36107.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAA83738.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAB01681.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAC03535.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAK53823.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence CAA48824.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in lysates from cells expressing human IL-13 recombinant protein.
Western Blot Analysis: 0.5 µg/mL of this antibody detected 10 µg of human IL-13 recombinant protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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