The TLS/FUS protein contains an RNA-recognition motif and is a component of nuclear riboprotein complexes. It resembles a transcription factor in that it binds DNA, contributes a transcriptional activation domain to the FUS-ERG oncoprotein, and interacts with several transcription factors in vitro. It binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. It may play a role in maintenance of genomic integrity.
Use Anti-TLS Antibody, clone 1FU-1D2 (Mouse Monoclonal Antibody) validated in WB, ICC to detect TLS also known as 75 kDa DNA-pairing protein, FUS-CHOP fusion protein.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected TLS in C2C12 cells.
This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq].
FUNCTION: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
SUBUNIT STRUCTURE: Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP.
SUBCELLULAR LOCATION: Nucleus
TISSUE SPECIFICITY: Ubiquitous.
PTM: Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
INVOLVEMENT IN DISEASE: A chromosomal aberration involving FUS is a cause of a form of malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
A chromosomal aberration involving FUS is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
SEQUENCE SIMILARITIES: Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected TLS on 10 µg of HepG2 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
R, H, M
ICC, WB
M
Ascites
Monoclonal Antibody
Description
Catalogue Number
04-1552
Description
Anti-TLS Antibody, clone 1FU-1D2
Alternate Names
translocated in liposarcoma
75 kDa DNA-pairing protein
FUS-CHOP fusion protein
FUS-CHOP protein fusion
Fusion gene in myxoid liposarcoma
Oncogene FUS
Oncogene TLS
Translocated in liposarcoma protein
fus-like protein
fus/tls-chop oncogene
fusion (involved in t(12
16) in malignant liposarcoma)
fusion, derived from t(12
16) malignant liposarcoma
heterogeneous nuclear ribonucleoprotein P2
heterogeneous nuclear ribonucleoprotein P2
Background Information
The TLS/FUS protein contains an RNA-recognition motif and is a component of nuclear riboprotein complexes. It resembles a transcription factor in that it binds DNA, contributes a transcriptional activation domain to the FUS-ERG oncoprotein, and interacts with several transcription factors in vitro. It binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. It may play a role in maintenance of genomic integrity.
Use Anti-TLS Antibody, clone 1FU-1D2 (Mouse Monoclonal Antibody) validated in WB, ICC to detect TLS also known as 75 kDa DNA-pairing protein, FUS-CHOP fusion protein.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected TLS in C2C12 cells.
This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq].
FUNCTION: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
SUBUNIT STRUCTURE: Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP.
SUBCELLULAR LOCATION: Nucleus
TISSUE SPECIFICITY: Ubiquitous.
PTM: Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.
INVOLVEMENT IN DISEASE: A chromosomal aberration involving FUS is a cause of a form of malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
A chromosomal aberration involving FUS is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%.
SEQUENCE SIMILARITIES: Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected TLS on 10 µg of HepG2 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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