nucleophosmin-retinoic acid receptor alpha fusion protein
NPM-RAR long form
NPM-RAR long form
retinoic acid receptor
Background Information
Retinoic acid receptor alpha (RARa) is a nuclear receptor encoded by the RARA gene. This gene is sometimes referred to as NR1B1 (nuclear receptor subfamily 1, group B, member 1). RARa is a member of the steroid/thyroid superfamily of nuclear receptor proteins that regulate gene expression by binding to RNA and other elements of DNA response. As implied by its name, this protein is a receptor for retinoic acid, a metabolite of vitamin A. Retinoic acid is necessary for normal organogenesis, but can act as a teratogen when present at high levels during embryonic and fetal development. During fetal and embryonic development retinoic acid regulates transcription through the activation of nuclear receptors, specifically the Retinoic Acid Receptor (RAR). Signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR), which form RXR/RAR heterodimers. There are three isoforms associated with RAR; RARα, RARβ and RARγ.
Product Information
Format
Ascites
Control
HL60 cell lysate
Presentation
Unpurified mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Applications
Application
Anti-RARα Antibody, clone 9α-9A6 is a Mouse Monoclonal Antibody for detection of RARα also known as Retinoic acid receptor alpha polypeptide & has been validated in WB, ICC.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RARα in HeLa and A431 cells.
Biological Information
Immunogen
Recombinant protein corresponding to human RARα.
Epitope
Unknown
Clone
clone 9α-9A6
Host
Mouse
Specificity
This antibody recognizes RARα.
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with human. Homology to mouse and rat is 91%.
Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 7468032]).[supplied by OMIM].
FUNCTION: This is a receptor for retinoic acid. This metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. This receptor controls cell function by directly regulating gene expression.
SIZE: 462 amino acids; 50771 Da
SUBUNIT: Interacts with CDK7 (By similarity). Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NOCA7 in a ligand- inducible manner.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
PTM: Phosphorylated. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity).
DISEASE: Chromosomal aberrations involving RARA may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM.
SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HL60 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected RARα on 10 µg of HL60 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
ICC, WB
M
Ascites
Monoclonal Antibody
Description
Catalogue Number
04-1545
Description
Anti-RARα Antibody, clone 9α-9A6
Alternate Names
retinoic acid receptor, alpha
Nuclear receptor subfamily 1 group B member 1
Retinoic acid receptor, alpha polypeptide
nucleophosmin-retinoic acid receptor alpha fusion protein
NPM-RAR long form
NPM-RAR long form
retinoic acid receptor
Background Information
Retinoic acid receptor alpha (RARa) is a nuclear receptor encoded by the RARA gene. This gene is sometimes referred to as NR1B1 (nuclear receptor subfamily 1, group B, member 1). RARa is a member of the steroid/thyroid superfamily of nuclear receptor proteins that regulate gene expression by binding to RNA and other elements of DNA response. As implied by its name, this protein is a receptor for retinoic acid, a metabolite of vitamin A. Retinoic acid is necessary for normal organogenesis, but can act as a teratogen when present at high levels during embryonic and fetal development. During fetal and embryonic development retinoic acid regulates transcription through the activation of nuclear receptors, specifically the Retinoic Acid Receptor (RAR). Signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR), which form RXR/RAR heterodimers. There are three isoforms associated with RAR; RARα, RARβ and RARγ.
Product Information
Format
Ascites
Control
HL60 cell lysate
Presentation
Unpurified mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Applications
Application
Anti-RARα Antibody, clone 9α-9A6 is a Mouse Monoclonal Antibody for detection of RARα also known as Retinoic acid receptor alpha polypeptide & has been validated in WB, ICC.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RARα in HeLa and A431 cells.
Biological Information
Immunogen
Recombinant protein corresponding to human RARα.
Epitope
Unknown
Clone
clone 9α-9A6
Host
Mouse
Specificity
This antibody recognizes RARα.
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Demonstrated to react with human. Homology to mouse and rat is 91%.
Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 7468032]).[supplied by OMIM].
FUNCTION: This is a receptor for retinoic acid. This metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. This receptor controls cell function by directly regulating gene expression.
SIZE: 462 amino acids; 50771 Da
SUBUNIT: Interacts with CDK7 (By similarity). Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NOCA7 in a ligand- inducible manner.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
PTM: Phosphorylated. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity).
DISEASE: Chromosomal aberrations involving RARA may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM.
SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HL60 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected RARα on 10 µg of HL60 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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