DNA excision repair protein ERCC-6-like, PLK1-interacting checkpoint helicase, Tumor antigen BJ-HCC-15
excision repair cross-complementing rodent repair deficiency, complementation group 6-like
excision repair cross-complementing rodent repair deficiency complementation group 6 - like
Tumor antigen BJ-HCC-15
PLK1-interacting checkpoint helicase
ATP-dependent helicase ERCC6-like
excision repair protein ERCC6-like
SNF2/RAD54 family protein
SNF2/RAD54 family protein
Background Information
Plk1-interacting checkpoint helicase (PICH ) is an essential component of the spindle assembly checkpoint (Baumann, C., et al. (2007). Cell 128:101–114). This protein is an ATPase family SNF2 member, and serves as a binding partner and substrate for Plk1. When PICH is phosphorylated, it retrieves Plk1 which serves to mediate the localization of PICH. As a DNA helicase, PICH gathers at kinetochores and centromeres during prometaphase, acting as a necessary part of the spindle assembly checkpoint by recruiting MAD2 and mediating chromatin centromeric tension. Depleted PICH results in the cancellation of the spindle checkpoint causing large scale missegregation of the chromosome.
Product Information
Format
Purified
Control
HeLa-S3 cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-PICCH Antibody, clone 142-26-3 (mouse monoclonal antibody) validated in WB, IP, ICC to detect PICCH also known as Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-dependent helicase ERCC6-like.
Key Applications
Immunocytochemistry
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC.
Biological Information
Immunogen
Histidine-tagged recombinant protein corresponding to human Plk1-interacting checkpoint helicase.
Epitope
Unknown
Clone
142-26-3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes Plk1-interacting checkpoint helicase.
FUNCTION: DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
SUBUNIT STRUCTURE; Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization.
SUBCELLULAR LOCATION: Centromere. Kinetochore. Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
PTM: Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 TPR repeats.
SEQUENCE CAUTION: The sequence AAM82750.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys.
The sequence BAA90952.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa-S3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
ICC, WB, IP
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-1540
Description
Anti-PICH Antibody, clone 142-26-3
Alternate Names
DNA excision repair protein ERCC-6-like, PLK1-interacting checkpoint helicase, Tumor antigen BJ-HCC-15
excision repair cross-complementing rodent repair deficiency, complementation group 6-like
excision repair cross-complementing rodent repair deficiency complementation group 6 - like
Tumor antigen BJ-HCC-15
PLK1-interacting checkpoint helicase
ATP-dependent helicase ERCC6-like
excision repair protein ERCC6-like
SNF2/RAD54 family protein
SNF2/RAD54 family protein
Background Information
Plk1-interacting checkpoint helicase (PICH ) is an essential component of the spindle assembly checkpoint (Baumann, C., et al. (2007). Cell 128:101–114). This protein is an ATPase family SNF2 member, and serves as a binding partner and substrate for Plk1. When PICH is phosphorylated, it retrieves Plk1 which serves to mediate the localization of PICH. As a DNA helicase, PICH gathers at kinetochores and centromeres during prometaphase, acting as a necessary part of the spindle assembly checkpoint by recruiting MAD2 and mediating chromatin centromeric tension. Depleted PICH results in the cancellation of the spindle checkpoint causing large scale missegregation of the chromosome.
Product Information
Format
Purified
Control
HeLa-S3 cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-PICCH Antibody, clone 142-26-3 (mouse monoclonal antibody) validated in WB, IP, ICC to detect PICCH also known as Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-dependent helicase ERCC6-like.
Key Applications
Immunocytochemistry
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC.
Biological Information
Immunogen
Histidine-tagged recombinant protein corresponding to human Plk1-interacting checkpoint helicase.
Epitope
Unknown
Clone
142-26-3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes Plk1-interacting checkpoint helicase.
FUNCTION: DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
SUBUNIT STRUCTURE; Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization.
SUBCELLULAR LOCATION: Centromere. Kinetochore. Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
PTM: Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 TPR repeats.
SEQUENCE CAUTION: The sequence AAM82750.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys.
The sequence BAA90952.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa-S3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
