CD86 (T-lymphocyte activation antigen CD86, activation B7-2 antigen or CTLA-4 counter-receptor B7.2) is a molecule expressed on antigen-presenting cells that provide costimulatory signals necessary for T cell activation and survival. CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4 (cytotoxic T-lymphocyte-associated protein 4). Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. Along with CD80, CD86 provides stimuli to prime T cells against antigens presented by antigen-presenting cells. CD86 is expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, macrophages, dendritic cells, and Langerhans cells, plus astrocytes. CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T and B cell-specific stimuli with a peak expression after 18-42 hours of culture. CD86 has also been shown to be involved in immunoglobulin class switching and triggering of mouse NK cell-mediated cytotoxicity.
Product Information
Format
Purified
Control
RAW264.7 cells
Presentation
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-CD86 Antibody, clone PO.3 is an antibody against CD86 for use in FC, IP, IH.
Key Applications
Immunohistochemistry
Flow Cytometry
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Nuriya, S., et al., 1996).
Imunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Nuriya, S., et al., 1996).
Biological Information
Immunogen
Mouse CD86 cDNA-transfected P815 cells.
Epitope
Unknown
Clone
PO.3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.
SUBUNIT STRUCTURE: Interacts with MARCH8.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICTY: Expressed on activated B-cells.
PTM: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
Flow Cytometry Analysis: 1 µg of this antibody detected CD86 in RAW264.7 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
CD86 (T-lymphocyte activation antigen CD86, activation B7-2 antigen or CTLA-4 counter-receptor B7.2) is a molecule expressed on antigen-presenting cells that provide costimulatory signals necessary for T cell activation and survival. CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4 (cytotoxic T-lymphocyte-associated protein 4). Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. Along with CD80, CD86 provides stimuli to prime T cells against antigens presented by antigen-presenting cells. CD86 is expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, macrophages, dendritic cells, and Langerhans cells, plus astrocytes. CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T and B cell-specific stimuli with a peak expression after 18-42 hours of culture. CD86 has also been shown to be involved in immunoglobulin class switching and triggering of mouse NK cell-mediated cytotoxicity.
Product Information
Format
Purified
Control
RAW264.7 cells
Presentation
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-CD86 Antibody, clone PO.3 is an antibody against CD86 for use in FC, IP, IH.
Key Applications
Immunohistochemistry
Flow Cytometry
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Nuriya, S., et al., 1996).
Imunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Nuriya, S., et al., 1996).
Biological Information
Immunogen
Mouse CD86 cDNA-transfected P815 cells.
Epitope
Unknown
Clone
PO.3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.
SUBUNIT STRUCTURE: Interacts with MARCH8.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICTY: Expressed on activated B-cells.
PTM: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
Flow Cytometry Analysis: 1 µg of this antibody detected CD86 in RAW264.7 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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