Chromatin assembly factor (CAF), is a heterotrimeric complex that belongs to the histone chaperone family. It is found in yeast, animals and plants, and facilitates the assembly of chromatin during DNA replication and repair. There are three CAF subunits: p48, p60, and p150. The p150 subunit interacts with heterochromatin protein 1, methyl-binding protein
1 and the Bloom’s syndrome DNA-helicase BLM. Mutations in CAF p150 have resulted in defects in embryo development.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-CAF1 p150 Antibody, clone SS1, 1-3 is a Mouse Monoclonal Antibody for detection of CAF1 p150 also known as Chromatin assembly factor I p150 subunit & has been validated in WB, ICC & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunocytochemistry
Application Notes
Western Blot Analysis: 1 µg/ml from a representative lot detected CAF p150 on 10 µg of HeLa cell lysate.
Biological Information
Immunogen
His-tagged recombinant human protein corresponding to CAF-1 p150.
Epitope
Unknown
Clone
clone SS1, 1-3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578])
FUNCTION: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci By similarity. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
SUBUNIT STRUCTURE: Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.
SUBCELLULAR LOCATION: Nucleus. Note: DNA replication foci.
DEVELOPMENTAL STAGE: Active complex is found in G1, S and G2 phases.
DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the CHAF1A family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected CAF on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
IP, WB, ICC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-1522
Description
Anti-CAF1 p150 Antibody, clone SS1, 1-3
Alternate Names
chromatin assembly factor I (150 kDa)
chromatin assembly factor 1, subunit A (p150)
Chromatin assembly factor I p150 subunit
CAF-I 150 kDa subunit
CAF-1 subunit A
subunit A (p150)
Background Information
Chromatin assembly factor (CAF), is a heterotrimeric complex that belongs to the histone chaperone family. It is found in yeast, animals and plants, and facilitates the assembly of chromatin during DNA replication and repair. There are three CAF subunits: p48, p60, and p150. The p150 subunit interacts with heterochromatin protein 1, methyl-binding protein
1 and the Bloom’s syndrome DNA-helicase BLM. Mutations in CAF p150 have resulted in defects in embryo development.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-CAF1 p150 Antibody, clone SS1, 1-3 is a Mouse Monoclonal Antibody for detection of CAF1 p150 also known as Chromatin assembly factor I p150 subunit & has been validated in WB, ICC & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunocytochemistry
Application Notes
Western Blot Analysis: 1 µg/ml from a representative lot detected CAF p150 on 10 µg of HeLa cell lysate.
Biological Information
Immunogen
His-tagged recombinant human protein corresponding to CAF-1 p150.
Epitope
Unknown
Clone
clone SS1, 1-3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578])
FUNCTION: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci By similarity. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
SUBUNIT STRUCTURE: Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.
SUBCELLULAR LOCATION: Nucleus. Note: DNA replication foci.
DEVELOPMENTAL STAGE: Active complex is found in G1, S and G2 phases.
DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the CHAF1A family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected CAF on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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