Replication protein A (RPA) is a multisubunit complex involved in DNA mismatch repair (MMR) in association with MSH2, MLH1 , PCNA, and DNA polymerase-delta. RPA is a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA1 p70 kDa is an ssDNA binding protein shown to be phosphorylated by ATM and ATR in response to DNA damage.RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-RPA1 p70 Antibody, clone RPA9, 1-30 (mouse monoclonal antibody) validated in WB, ICC to detect RPA1 p70 also known as RF-A protein 1, RP-A p70, Replication factor A protein 1, REPA1, RPA1, HSSB.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry
Preformed by an external laboratory using representative lot of this antibody.
Biological Information
Immunogen
His-tagged recombinant protein corresponding to human RPA1.
Clone
RPA9, 1-30
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: It participates in a very early step in initiation. RP-A is a single-stranded DNA-binding protein. Absolutely required for simian virus 40 DNA replication in vitro.
SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RIP and XPA. Interacts with RPA4.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the replication factor A protein 1 family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected RPA1 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
ICC, WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-1521
Description
Anti-RPA1 p70 Antibody, clone RPA9, 1-30
Alternate Names
HSSB.
RF-A protein 1
RP-A p70
Replication factor A protein 1
Single-stranded DNA-binding protein
replication protein A 70 kDa DNA-binding subunit
replication protein A1 (70kD)
replication protein A1, 70kDa
REPA1
RPA1
RPA1
Background Information
Replication protein A (RPA) is a multisubunit complex involved in DNA mismatch repair (MMR) in association with MSH2, MLH1 , PCNA, and DNA polymerase-delta. RPA is a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA1 p70 kDa is an ssDNA binding protein shown to be phosphorylated by ATM and ATR in response to DNA damage.RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-RPA1 p70 Antibody, clone RPA9, 1-30 (mouse monoclonal antibody) validated in WB, ICC to detect RPA1 p70 also known as RF-A protein 1, RP-A p70, Replication factor A protein 1, REPA1, RPA1, HSSB.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry
Preformed by an external laboratory using representative lot of this antibody.
Biological Information
Immunogen
His-tagged recombinant protein corresponding to human RPA1.
Clone
RPA9, 1-30
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: It participates in a very early step in initiation. RP-A is a single-stranded DNA-binding protein. Absolutely required for simian virus 40 DNA replication in vitro.
SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RIP and XPA. Interacts with RPA4.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the replication factor A protein 1 family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected RPA1 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
