Siglec-8 is a sialic acid-binding immunoglobulin-like lectin that is mostly expressed in eosinophils. Siglec-8 is thought to contain three immunoglobulin-like domains, a transmembrane region, and a 47 amino acid cytoplasmic tail. Siglec-8 is expressed on COS cells and is able to intercede sialic acid-dependent binding to erythrocytes and sialoglycoconjugates. Combining Siglec-8 with certain antibodies is thought to promote human eosinophil apoptosis.
Product Information
Format
Purified
Control
Human PBMC's
Presentation
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Detect Siglec-8 using this Anti-Siglec-8 Antibody, clone 7C9 validated for use in FC.
Key Applications
Flow Cytometry
Application Notes
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA (Floyd, H., et al. (2000). Journal of Biological Chemistry. 275(2):861–866).
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Floyd, H., et al. (2000). Journal of Biological Chemistry. 275(2):861–866).
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Floyd, H., et al. (2000). Journal of Biological Chemistry. 275(2):861–866).
Biological Information
Immunogen
Extracellular region of human recombinant Siglec-8 cleaved with Factor Xa.
Clone
7C9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM].
FUNCTION: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICTY: Expressed specifically on eosinophils.
DOMAIN: Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
SEQUENCE SIMILARITIES: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Flow Cytometry Analysis: 2 µg of this antibody detected Siglec-8 in human PBMC's.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
FC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-1473
Description
Anti-Siglec-8 Antibody, clone 7C9
Alternate Names
Sialoadhesin family member 2
sialic acid binding Ig-like lectin 8
sialic acid-binding Ig-like lectin 8
CD329 antigen
Background Information
Siglec-8 is a sialic acid-binding immunoglobulin-like lectin that is mostly expressed in eosinophils. Siglec-8 is thought to contain three immunoglobulin-like domains, a transmembrane region, and a 47 amino acid cytoplasmic tail. Siglec-8 is expressed on COS cells and is able to intercede sialic acid-dependent binding to erythrocytes and sialoglycoconjugates. Combining Siglec-8 with certain antibodies is thought to promote human eosinophil apoptosis.
Product Information
Format
Purified
Control
Human PBMC's
Presentation
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Detect Siglec-8 using this Anti-Siglec-8 Antibody, clone 7C9 validated for use in FC.
Key Applications
Flow Cytometry
Application Notes
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA (Floyd, H., et al. (2000). Journal of Biological Chemistry. 275(2):861–866).
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Floyd, H., et al. (2000). Journal of Biological Chemistry. 275(2):861–866).
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Floyd, H., et al. (2000). Journal of Biological Chemistry. 275(2):861–866).
Biological Information
Immunogen
Extracellular region of human recombinant Siglec-8 cleaved with Factor Xa.
Clone
7C9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM].
FUNCTION: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICTY: Expressed specifically on eosinophils.
DOMAIN: Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
SEQUENCE SIMILARITIES: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Flow Cytometry Analysis: 2 µg of this antibody detected Siglec-8 in human PBMC's.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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