ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion/migration. The kinase activity appears to be reduced following Integrin activation, and overexpression of p59ILK inhibits cellular adhesion to Integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).
Product Information
Format
Culture Supernatant
Control
HeLa, 293T and Jurkat cell lysates
Presentation
Cell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-ILK Antibody, clone EPR1592, Rabbit is validated for use in WB, IC, FC for the detection of ILK.
Key Applications
Flow Cytometry
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: 1:100-250 dilution from a previous lot detected ILK in HeLa cell lysate.
Flow Cytometry: 1:60 dilution from a previous lot of this antibody was used in FC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues in human ILK.
FUNCTION:Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
CATALYTIC ACTIVITY:ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION:Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.
SUBUNIT STRUCTURE:Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1.
TISSUE SPECIFICITY:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
DOMAIN:A PH-like domain is involved in phosphatidylinositol phosphate binding.
PTM:Autophosphorylated on serine residues.
SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on HeLa, 293T and Jurkat cell lysates.
Western Blot Analysis: 1:5,000 - 50,000 dilution of this antibody was used to detect ILK in HeLa, 293T and Jurkat cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion/migration. The kinase activity appears to be reduced following Integrin activation, and overexpression of p59ILK inhibits cellular adhesion to Integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).
Product Information
Format
Culture Supernatant
Control
HeLa, 293T and Jurkat cell lysates
Presentation
Cell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-ILK Antibody, clone EPR1592, Rabbit is validated for use in WB, IC, FC for the detection of ILK.
Key Applications
Flow Cytometry
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: 1:100-250 dilution from a previous lot detected ILK in HeLa cell lysate.
Flow Cytometry: 1:60 dilution from a previous lot of this antibody was used in FC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues in human ILK.
FUNCTION:Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
CATALYTIC ACTIVITY:ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION:Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.
SUBUNIT STRUCTURE:Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1.
TISSUE SPECIFICITY:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
DOMAIN:A PH-like domain is involved in phosphatidylinositol phosphate binding.
PTM:Autophosphorylated on serine residues.
SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on HeLa, 293T and Jurkat cell lysates.
Western Blot Analysis: 1:5,000 - 50,000 dilution of this antibody was used to detect ILK in HeLa, 293T and Jurkat cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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