收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

Anti-phospho-Her4/ErbB-4 (Tyr1258),clone

  • 产品编号:merck-m-04-1144      品牌:merck-millipore       原厂货号:04-1144
  • 产品分类:抗体 > 一抗 > 磷酸化抗体
  • 应用分类:
 
包装: EA
运保温度: -20ºC
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

描述:

Key Spec Table

Species Reactivity Key Applications Host Format Antibody Type
H WB Rb Unpurified Monoclonal Antibody

Description 
Catalogue Number 04-1144
Description Anti-phospho-Her4/ErbB-4 (Tyr1258) Antibody, clone EP2272AY, rabbit monoclonal
Alternate Names
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
  • Tyrosine kinase-type cell surface receptor HER4
  • avian erythroblastic leukemia viral (v-erb-b2) oncogene<br />homolog 4
  • receptor tyrosine-protein kinase erbB-4
  • v-erb-a avian erythroblastic leukemia viral oncogene<br />homolog-like 4
Background Information ErbB4/Her4 is a member of the ErbB receptor family of tyrosine kinases. It is largely expressed in many breast carcinoma cell lines; however, it is also found in normal skeletal muscle, heart, pituitary, brain, and cerebellum. This receptor is more specified than the others in this family because of its unique ability of the C-terminal to bind to a PDZ domain.

Product Information 
Format Unpurified
Control
  • SKBR3 cell lysate
Presentation Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Applications 
Application This Anti-phospho-Her4/ErbB-4 (Tyr1258) Antibody, clone EP2272AY, Rabbit is validated for use in WB for the detection of phospho-Her4/ErbB-4 (Tyr1258).
Key Applications
  • Western Blotting

Biological Information 
Immunogen Phospho-specific peptide corresponding to residues surrounding Tyr1258 of human Her4/ErbB-4.
Epitope Phosphorylated Tyr1258
Clone EP2272AY
Host Rabbit
Specificity This antibody detects Her4/ErbB-4 phosphorylated on Tyr258.
Isotype IgG
Species Reactivity Human
Species Reactivity Note Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • p180erbB4
  • EC 2.7.10.1
  • HER4
  • MGC138404
Modifications
  • Phosphorylation
Purification Method Unpurified
UniProt Number
UniProt Summary FUNCTION: Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Homodimer or heterodimer with each of the other ERBB receptors Potential. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with CBFA2T3, MUC1 and WWOX. 
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. 
TISSUE SPECIFITIY: Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A and isoform JM-B are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
DOMAIN: The WW-binding motifs mediate interaction with WWOX. 
PTM: Isoform JM-A is processed but not isoform JM-B. So, they respectively represent cleavable and non-cleavable forms of the receptor.
Ligand-binding increases phosphorylation on tyrosine residues.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.

Product Usage Statements 
Quality Assurance Evaluated by Western Blot on SKBR3 cell lysates.
Western Blot Analysis: A 1:1,000-5,000 dilution of this antibody was used to detect Her4/ErbB-4 on SKBR3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information 
Storage Conditions Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information 
Material Size 100 µL

 


相关图片

说明书

参考文献

本产品可用于的实验

购此产品的人还购买了
最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.21

 纠 错