Integrin alpha4 (CD49d, VLA-4) is a member of the integrin superfamily of adhesion receptors. Integrin alpha4 inhibits cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, where other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (Teixido, 1992). Integrin alpha4 is involved in embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (Liu, 1999).
Product Information
Format
Culture Supernatant
Control
Jurkat cell lysate
Presentation
Cell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Integrin alpha4 using this Anti-Integrin alpha4 Antibody, clone EPR1355Y, Rabbit validated for use in WB, IC, IH(P) & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected InImmunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected Integrin alpha4 in human tonsil tissue.
Immunocytochemistry Analysis: 1:50 dilution from a previous lot detected Integrin alpha4 in HeLa cell lysate.
Immunoprecipitation: 1:40 dilution from a previous lot was used on IP.
tegrin alpha4 in human tonsil tissue.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human Integrin alpha4.
FUNCTION:Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
SUBUNIT STRUCTURE:Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.
SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein.
DOMAIN:The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
PTM:Phosphorylation on Ser-1027 inhibits PXN binding.
SEQUENCE SIMILARITIES:Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
SEQUENCE CAUTION:The sequence AAB59613.1 differs from that shown. Reason: Frameshift at position 13.
The sequence CAA34852.1 differs from that shown. Reason: Frameshift at position 13.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Jurkat cell lysate.
Western Blot Analysis; 1:1,000 - 2,000 dilution of this antibody was used to detect Integrin alpha4 in Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Integrin alpha4 (CD49d, VLA-4) is a member of the integrin superfamily of adhesion receptors. Integrin alpha4 inhibits cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, where other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (Teixido, 1992). Integrin alpha4 is involved in embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (Liu, 1999).
Product Information
Format
Culture Supernatant
Control
Jurkat cell lysate
Presentation
Cell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Integrin alpha4 using this Anti-Integrin alpha4 Antibody, clone EPR1355Y, Rabbit validated for use in WB, IC, IH(P) & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected InImmunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected Integrin alpha4 in human tonsil tissue.
Immunocytochemistry Analysis: 1:50 dilution from a previous lot detected Integrin alpha4 in HeLa cell lysate.
Immunoprecipitation: 1:40 dilution from a previous lot was used on IP.
tegrin alpha4 in human tonsil tissue.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human Integrin alpha4.
FUNCTION:Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
SUBUNIT STRUCTURE:Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.
SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein.
DOMAIN:The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
PTM:Phosphorylation on Ser-1027 inhibits PXN binding.
SEQUENCE SIMILARITIES:Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
SEQUENCE CAUTION:The sequence AAB59613.1 differs from that shown. Reason: Frameshift at position 13.
The sequence CAA34852.1 differs from that shown. Reason: Frameshift at position 13.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Jurkat cell lysate.
Western Blot Analysis; 1:1,000 - 2,000 dilution of this antibody was used to detect Integrin alpha4 in Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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