Anti-Casein Kinase II (beta subunit) Antibody, clone EP1995Y, rabbit monoclonal
Alternate Names
casein kinase 2, beta polypeptide
CK II beta
Casein kinase II beta subunit
alternative name: G5a, phosvitin
phosvitin
casein kinase 2
Background Information
Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer.
Product Information
Format
Unpurified
Control
K562 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
Applications
Application
Anti-Casein Kinase II (beta subunit) Antibody, clone EP1995Y, Rabbit detects level of Casein Kinase II (beta subunit) & has been published & validated for use in WB, FC, IH(P) & IC.
Key Applications
Immunocytochemistry
Western Blotting
Flow Cytometry
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Casein Kinase II (beta subunit) in human ovary carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected Casein Kinase II (beta subunit) in HeLa cells.
Flow Cytometry: A 1:20 dilution of a previous lot was used in flow cytometry.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human Casein Kinase II (beta subunit).
Epitope
C-terminus
Clone
EP1995Y
Host
Rabbit
Specificity
This antibody recognizes Casein Kinase II (beta subunit).
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus.
FUNCTION:Participates in Wnt signaling By similarity. Plays a complex role in regulating the basal catalytic activity of the alpha subunit. SUBUNIT STRUCTURE:Tetramer composed of an alpha subunit, an alpha' subunit and two beta subunits. Interacts with TCTEX1D3 By similarity. Interacts with CD163. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. PTM:Phosphorylated by alpha subunit.SEQEUNCE SIMILARITIES:Belongs to the casein kinase 2 subunit beta family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on K562 cell lysates.
Western Blot Analysis: A 1:500-2,000 dilution of this antibody was used to detect Casein Kinase II (beta subunit) in K562 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
R, H, M
ICC, WB, FC, IH(P)
Rb
Unpurified
Monoclonal Antibody
Description
Catalogue Number
04-1128
Description
Anti-Casein Kinase II (beta subunit) Antibody, clone EP1995Y, rabbit monoclonal
Alternate Names
casein kinase 2, beta polypeptide
CK II beta
Casein kinase II beta subunit
alternative name: G5a, phosvitin
phosvitin
casein kinase 2
Background Information
Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer.
Product Information
Format
Unpurified
Control
K562 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
Applications
Application
Anti-Casein Kinase II (beta subunit) Antibody, clone EP1995Y, Rabbit detects level of Casein Kinase II (beta subunit) & has been published & validated for use in WB, FC, IH(P) & IC.
Key Applications
Immunocytochemistry
Western Blotting
Flow Cytometry
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Casein Kinase II (beta subunit) in human ovary carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected Casein Kinase II (beta subunit) in HeLa cells.
Flow Cytometry: A 1:20 dilution of a previous lot was used in flow cytometry.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human Casein Kinase II (beta subunit).
Epitope
C-terminus
Clone
EP1995Y
Host
Rabbit
Specificity
This antibody recognizes Casein Kinase II (beta subunit).
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus.
FUNCTION:Participates in Wnt signaling By similarity. Plays a complex role in regulating the basal catalytic activity of the alpha subunit. SUBUNIT STRUCTURE:Tetramer composed of an alpha subunit, an alpha' subunit and two beta subunits. Interacts with TCTEX1D3 By similarity. Interacts with CD163. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. PTM:Phosphorylated by alpha subunit.SEQEUNCE SIMILARITIES:Belongs to the casein kinase 2 subunit beta family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on K562 cell lysates.
Western Blot Analysis: A 1:500-2,000 dilution of this antibody was used to detect Casein Kinase II (beta subunit) in K562 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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