sirtuin (silent mating type information regulation 2<br />homolog) 2 (S. cerevisiae)
sirtuin (silent mating type information regulation 2,<br />S.cerevisiae, homolog) 2
sirtuin 2
sirtuin 2
Background Information
Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.
Product Information
Format
Unpurified
Control
SKBR-3 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications
Flow Cytometry
Immunoprecipitation
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected SIRT2 in PC12 cells.
Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of human SIRT2.
Epitope
C-terminus
Clone
EP1668Y
Host
Rabbit
Specificity
This antibody recognizes SIRT2 at and around the C-terminus, all three isoforms.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.
FUNCTION:NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo. CATALYTIC ACTIVITY:NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR:Binds 1 zinc ion per subunit. ENZYME REGULATION:Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. SUBUNIT STRUCTURE:Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. SUBCELLULAR LOCATION:Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules. TISSUE SPECIFICITY:Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. DEVELOPMENTAL STAGE:Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. PTM:Phosphorylated at the G2/M transition of the cell cycle. SEQUENCE SIMILARITIES:Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. SEQUENCE CAUTION:The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on SKBR-3 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect SIRT2 in SKBR-3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
sirtuin (silent mating type information regulation 2<br />homolog) 2 (S. cerevisiae)
sirtuin (silent mating type information regulation 2,<br />S.cerevisiae, homolog) 2
sirtuin 2
sirtuin 2
Background Information
Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.
Product Information
Format
Unpurified
Control
SKBR-3 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications
Flow Cytometry
Immunoprecipitation
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected SIRT2 in PC12 cells.
Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of human SIRT2.
Epitope
C-terminus
Clone
EP1668Y
Host
Rabbit
Specificity
This antibody recognizes SIRT2 at and around the C-terminus, all three isoforms.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.
FUNCTION:NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo. CATALYTIC ACTIVITY:NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR:Binds 1 zinc ion per subunit. ENZYME REGULATION:Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. SUBUNIT STRUCTURE:Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. SUBCELLULAR LOCATION:Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules. TISSUE SPECIFICITY:Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. DEVELOPMENTAL STAGE:Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. PTM:Phosphorylated at the G2/M transition of the cell cycle. SEQUENCE SIMILARITIES:Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. SEQUENCE CAUTION:The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on SKBR-3 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect SIRT2 in SKBR-3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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