CD44 antigen (homing function and Indian blood groupsystem)
CD44 molecule (Indian blood group)
CDW44 antigen
Extracellular matrix receptor III
GP90 lymphocyte homing/adhesion receptor
Heparan sulfate proteoglycan
Hermes antigen
Hyaluronate receptor
Phagocytic glycoprotein I
antigen gp90 homing receptor
cell adhesion molecule
cell surface glycoprotein CD44
chondroitin sulfate proteoglycan 8
extracellular matrix receptor-III
hematopoietic cell E- and L-selectin ligand
Background Information
Recent studies indicate that the expression of CD44 (H-CAM) on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas. Sequencing analysis reveals that CD44 exists in different forms (at least 15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s. Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced. In particular, CD44s contains exons 1-15 and exons 15-19. The other exons (e.g. 6-14) are also spliced in a variety of different cells. It has been suggested that expression of CD44 variants with the additional exon 14 (so-called v10) is responsible for tumor progression and metastasis. In fact, CD44v10 expression has been used as an indicator of metastasis.
Product Information
Format
Unpurified
Control
TF-1 cell lysate.
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-CD44 [H-CAM] Antibody, clone EPR1013Y, Rabbit detects level of CD44 [H-CAM] & has been published & validated for use in IH(P), WB, IC, FC.
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry
Flow Cytometry
Application Notes
Immunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected CD44 in human urinary bladder tissue.
Immunocytochemistry: 1:100-250 dilution from a previous lot of this antibody was used in IC.
Flow Cytometry: 1:30 dilution from a previous lot of this antibody was used in FC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues in human CD44.
FUNCTION:Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. SUBUNIT STRUCTURE:Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY:An epithelial CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells. PTM:Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. POLYMORPHISM:CD44 is responsible for the Indian blood group system. The molecular basis of the In(A)=In1/In(B)=In2 blood group antigens is a single variation in position 46; In(B), the most frequent allele, has Arg-46. SEQUENCE SIMILARITIES:Contains 1 Link domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on TF-1 cell lysate.
Western Blot Analysis: 1:5,000 dilution of this antibody was used to detect CD44 in TF-1 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
CD44 antigen (homing function and Indian blood groupsystem)
CD44 molecule (Indian blood group)
CDW44 antigen
Extracellular matrix receptor III
GP90 lymphocyte homing/adhesion receptor
Heparan sulfate proteoglycan
Hermes antigen
Hyaluronate receptor
Phagocytic glycoprotein I
antigen gp90 homing receptor
cell adhesion molecule
cell surface glycoprotein CD44
chondroitin sulfate proteoglycan 8
extracellular matrix receptor-III
hematopoietic cell E- and L-selectin ligand
Background Information
Recent studies indicate that the expression of CD44 (H-CAM) on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas. Sequencing analysis reveals that CD44 exists in different forms (at least 15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s. Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced. In particular, CD44s contains exons 1-15 and exons 15-19. The other exons (e.g. 6-14) are also spliced in a variety of different cells. It has been suggested that expression of CD44 variants with the additional exon 14 (so-called v10) is responsible for tumor progression and metastasis. In fact, CD44v10 expression has been used as an indicator of metastasis.
Product Information
Format
Unpurified
Control
TF-1 cell lysate.
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-CD44 [H-CAM] Antibody, clone EPR1013Y, Rabbit detects level of CD44 [H-CAM] & has been published & validated for use in IH(P), WB, IC, FC.
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Immunocytochemistry
Flow Cytometry
Application Notes
Immunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected CD44 in human urinary bladder tissue.
Immunocytochemistry: 1:100-250 dilution from a previous lot of this antibody was used in IC.
Flow Cytometry: 1:30 dilution from a previous lot of this antibody was used in FC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues in human CD44.
FUNCTION:Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. SUBUNIT STRUCTURE:Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY:An epithelial CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells. PTM:Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. POLYMORPHISM:CD44 is responsible for the Indian blood group system. The molecular basis of the In(A)=In1/In(B)=In2 blood group antigens is a single variation in position 46; In(B), the most frequent allele, has Arg-46. SEQUENCE SIMILARITIES:Contains 1 Link domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on TF-1 cell lysate.
Western Blot Analysis: 1:5,000 dilution of this antibody was used to detect CD44 in TF-1 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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