sirtuin (silent mating type information regulation 2
homolog) 2 (S. cerevisiae)
sirtuin (silent mating type information regulation 2,
S.cerevisiae, homolog) 2
sirtuin 2
sirtuin 2
Background Information
Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein.
Product Information
Format
Unpurified
Control
Jurkat cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Sir2 in human colon and lung squamous carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2.
Epitope
C-terminus
Clone
E104
Host
Rabbit
Specificity
The antibody does not cross-react with other sirtuin family members.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
FUNCTION:NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo. CATALYTIC ACTIVITY:NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR:Binds 1 zinc ion per subunit. ENZYME REGULATION:Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. SUBUNIT STRUCTURE:Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. SUBCELLULAR LOCATION:Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules. TISSUE SPECIFICITY:Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. DEVELOPMENTAL STAGE:Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. PTM:Phosphorylated at the G2/M transition of the cell cycle. SEQUENCE SIMILARITIES:Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. SEQUENCE CAUTION:The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Jurkat cell lysates.
Western Blot Analysis: A 1:5,000 dilution of this antibody was used to detect Sir2 in Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
sirtuin (silent mating type information regulation 2
homolog) 2 (S. cerevisiae)
sirtuin (silent mating type information regulation 2,
S.cerevisiae, homolog) 2
sirtuin 2
sirtuin 2
Background Information
Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein.
Product Information
Format
Unpurified
Control
Jurkat cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Sir2 in human colon and lung squamous carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2.
Epitope
C-terminus
Clone
E104
Host
Rabbit
Specificity
The antibody does not cross-react with other sirtuin family members.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
FUNCTION:NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo. CATALYTIC ACTIVITY:NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR:Binds 1 zinc ion per subunit. ENZYME REGULATION:Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. SUBUNIT STRUCTURE:Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. SUBCELLULAR LOCATION:Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules. TISSUE SPECIFICITY:Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. DEVELOPMENTAL STAGE:Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. PTM:Phosphorylated at the G2/M transition of the cell cycle. SEQUENCE SIMILARITIES:Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. SEQUENCE CAUTION:The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Jurkat cell lysates.
Western Blot Analysis: A 1:5,000 dilution of this antibody was used to detect Sir2 in Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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