Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal
Alternate Names
calcium/calmodulin-dependent protein kinase type IV<br />catalytic chain
CAM kinase IV
CAM kinase- GR
CAM kinase-GR
CaMK IV
brain Ca(2+)-calmodulin-dependent protein kinase type IV
brain Ca++-calmodulin-dependent protein kinase type IV
calcium/calmodulin-dependent protein kinase IV
Background Information
The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.
Product Information
Format
Unpurified
Control
Jurkat and Human brain lysates
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, Rabbit is an antibody against Calcium/Calmodulin Kinase IV for use in WB & IC.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the N-terminus of human Calcium/Calmodulin Kinase IV.
Epitope
N-terminus
Clone
EP2564Y
Host
Rabbit
Specificity
This antibody recognizes Calcium/Calmodulin Kinase IV.
The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells
FUNCTION:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory By similarity. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION:Activated by Ca2+/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca2+/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca2+/calmodulin-independent state By similarity. Probably inactivated by serine/threonine protein phosphatase 2A.
SUBUNIT STRUCTURE:Monomer By similarity. Interacts with serine/threonine protein phosphatase 2A catalytic subunit, PPP2CA or PPP2CB. The interaction with PP2CA or PP2CB is mutually exclusive with binding to Ca2+/calmodulin.
SUBCELLULAR LOCATION:Cytoplasm By similarity. Nucleus By similarity. Note: Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix By similarity. TISSUE SPECIFICTY:Expressed in epithelial ovarian cancer tissue. Ref.8 PTM:Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 By similarity. Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Jurkat and Human brain lysates.
Western Blot Analysis: A 1:1,000-2,000 dilution of this antibody was used to detect Calcium/Calmodulin Kinase IV in Jurkat and Human brain lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
R, H, M
ICC, WB
Rb
Unpurified
Monoclonal Antibody
Description
Catalogue Number
04-1081
Description
Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, rabbit monoclonal
Alternate Names
calcium/calmodulin-dependent protein kinase type IV<br />catalytic chain
CAM kinase IV
CAM kinase- GR
CAM kinase-GR
CaMK IV
brain Ca(2+)-calmodulin-dependent protein kinase type IV
brain Ca++-calmodulin-dependent protein kinase type IV
calcium/calmodulin-dependent protein kinase IV
Background Information
The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.
Product Information
Format
Unpurified
Control
Jurkat and Human brain lysates
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Anti-Calcium/Calmodulin Kinase IV Antibody, clone EP2564Y, Rabbit is an antibody against Calcium/Calmodulin Kinase IV for use in WB & IC.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the N-terminus of human Calcium/Calmodulin Kinase IV.
Epitope
N-terminus
Clone
EP2564Y
Host
Rabbit
Specificity
This antibody recognizes Calcium/Calmodulin Kinase IV.
The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells
FUNCTION:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory By similarity. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION:Activated by Ca2+/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2. Autophosphorylation of the N-terminus is required for full activation. In part, activity is independent on Ca2+/calmodulin and autophosphorylation of Ser-336 allows to switch to a Ca2+/calmodulin-independent state By similarity. Probably inactivated by serine/threonine protein phosphatase 2A.
SUBUNIT STRUCTURE:Monomer By similarity. Interacts with serine/threonine protein phosphatase 2A catalytic subunit, PPP2CA or PPP2CB. The interaction with PP2CA or PP2CB is mutually exclusive with binding to Ca2+/calmodulin.
SUBCELLULAR LOCATION:Cytoplasm By similarity. Nucleus By similarity. Note: Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix By similarity. TISSUE SPECIFICTY:Expressed in epithelial ovarian cancer tissue. Ref.8 PTM:Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 By similarity. Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Jurkat and Human brain lysates.
Western Blot Analysis: A 1:1,000-2,000 dilution of this antibody was used to detect Calcium/Calmodulin Kinase IV in Jurkat and Human brain lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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