GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
Product Information
Format
Unpurified
Control
293T cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-GSK3 β (Ser9) using this Anti-phospho-GSK3 β (Ser9) Antibody, clone EPR2286Y, Rabbit validated for use in WB, IH(P) & IC.
Key Applications
Immunocytochemistry
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected GSK3 β in human ovarian carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot worked in IC.
Biological Information
Immunogen
Phospho-specific peptide corresponding to residues surrounding Ser9 of human GSK3 β.
Epitope
Phosphorylated Ser9
Clone
EPR2286Y
Host
Rabbit
Specificity
The antibody only detects GSK3 β phosphorylated on Ser9.
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).
FUNCTION:Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Ref.5 CATALYTIC ACTIVITY:ATP + [tau protein] = ADP + [tau protein] phosphate. ENZYME REGULATION:Inhibited when phosphorylated by AKT1. SUBUNIT STRUCTURE:Monomer By similarity. Interacts with CABYR, MUC1, NIN and PRUNE. TISSUE SPECIFICITY:Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. PTM:Phosphorylated by AKT1 and ILK1. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in untreated and calyculin-treated 293T cell lysates.
Western Blot Analysis: A 1:10,000-20,000 dilution of this antibody was used to detect GSK3 β in calyculin-treated 293T cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
Product Information
Format
Unpurified
Control
293T cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-GSK3 β (Ser9) using this Anti-phospho-GSK3 β (Ser9) Antibody, clone EPR2286Y, Rabbit validated for use in WB, IH(P) & IC.
Key Applications
Immunocytochemistry
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected GSK3 β in human ovarian carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot worked in IC.
Biological Information
Immunogen
Phospho-specific peptide corresponding to residues surrounding Ser9 of human GSK3 β.
Epitope
Phosphorylated Ser9
Clone
EPR2286Y
Host
Rabbit
Specificity
The antibody only detects GSK3 β phosphorylated on Ser9.
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).
FUNCTION:Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Ref.5 CATALYTIC ACTIVITY:ATP + [tau protein] = ADP + [tau protein] phosphate. ENZYME REGULATION:Inhibited when phosphorylated by AKT1. SUBUNIT STRUCTURE:Monomer By similarity. Interacts with CABYR, MUC1, NIN and PRUNE. TISSUE SPECIFICITY:Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. PTM:Phosphorylated by AKT1 and ILK1. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in untreated and calyculin-treated 293T cell lysates.
Western Blot Analysis: A 1:10,000-20,000 dilution of this antibody was used to detect GSK3 β in calyculin-treated 293T cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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