In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. MSI2 or Musashi2 is a member of RNA-binding proteins that appear to play central roles in post transcriptional gene regulation. The MSI2 gene encodes a protein containing two conserved tandem RNA recognition motifs. MSI2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages.
Product Information
Format
Unpurified
Control
Human brain, Rat brain, SW480, and T47D cell lysates
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Musashi-2 Antibody, clone EP1305Y, Rabbit is validated for use in WB, IH(P), FC for the detection of Musashi-2.
Key Applications
Flow Cytometry
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry (Paraffin) Analysis:
Representative lot data.
Immunohistochemical staining of paraffin-embedded human placenta tissue using 1:100-1:250 dilution of anti-Musashi-2, clone EP1305Y.
Flow Cytometry: 1:30 dilution of a previous lot was used in flow cytometry.
Biological Information
Immunogen
A synthetic peptide derived from Human MSI2 (UniProt: Q96DH6). (The immunogen is located within the sequence of amino acids 240-280, UniProt accession ID Q96DH6). Based on the immunogen sequence for this antibody, it is not predicted to detect the shorter isoforms of MSI2
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene.
FUNCTION:RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity. SUBCELLULAR LOCATION:Cytoplasm. Note: Associated with polysomes By similarity. TISSUE SPECIFICITY:Ubiquitous; detected at low levels. INDUCTION:Up-regulated in astrocytes after brain injury By similarity. PTM:Phosphorylated By similarity. INVOLVEMENT IN DISEASE:Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23). SEQUENCE SIMILARITIES:Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Human brain, Rat brain, SW480, and T47D cell lysates.
Western Blot Analysis: 1:1,000-2,000 dilution of this antibody was used to detect Musashi-2 in human brain, rat brain, SW480, and T47D cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. MSI2 or Musashi2 is a member of RNA-binding proteins that appear to play central roles in post transcriptional gene regulation. The MSI2 gene encodes a protein containing two conserved tandem RNA recognition motifs. MSI2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages.
Product Information
Format
Unpurified
Control
Human brain, Rat brain, SW480, and T47D cell lysates
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Musashi-2 Antibody, clone EP1305Y, Rabbit is validated for use in WB, IH(P), FC for the detection of Musashi-2.
Key Applications
Flow Cytometry
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry (Paraffin) Analysis:
Representative lot data.
Immunohistochemical staining of paraffin-embedded human placenta tissue using 1:100-1:250 dilution of anti-Musashi-2, clone EP1305Y.
Flow Cytometry: 1:30 dilution of a previous lot was used in flow cytometry.
Biological Information
Immunogen
A synthetic peptide derived from Human MSI2 (UniProt: Q96DH6). (The immunogen is located within the sequence of amino acids 240-280, UniProt accession ID Q96DH6). Based on the immunogen sequence for this antibody, it is not predicted to detect the shorter isoforms of MSI2
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene.
FUNCTION:RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity. SUBCELLULAR LOCATION:Cytoplasm. Note: Associated with polysomes By similarity. TISSUE SPECIFICITY:Ubiquitous; detected at low levels. INDUCTION:Up-regulated in astrocytes after brain injury By similarity. PTM:Phosphorylated By similarity. INVOLVEMENT IN DISEASE:Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23). SEQUENCE SIMILARITIES:Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Human brain, Rat brain, SW480, and T47D cell lysates.
Western Blot Analysis: 1:1,000-2,000 dilution of this antibody was used to detect Musashi-2 in human brain, rat brain, SW480, and T47D cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
