Glutamate is a widely utilized excitatory neurotransmitter in mammalian brain. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate (KA) and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are referred to as non-NMDA receptors. Two distinct genes encode the NMDA receptor subunits, NR1 and NR2. The NR1 subunit is expressed in abundance in virtually all regions of the brain.
Product Information
Format
Unpurified
Control
Mouse brain cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
This Anti-phospho-NMDAR1(Ser889) Antibody, clone EPR2480Y, Rabbit is validated for use in WB, IH(P), IC, IP for the detection of phospho-NMDAR1(Ser889).
Key Applications
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected NMDAR1 in human brain tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:30 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Phospho-specific peptide corresponding to residues surrounding Ser889 of human NMDAR1.
Epitope
Phosphorylated Ser889
Clone
EPR2480Y
Host
Rabbit
Specificity
This antibody detects NMDAR1 phosphorylated at Ser889.
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.
FUNCTION:NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors By similarity. SUBUNIT STRUCTURE:Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ By similarity. SUBCELLULAR LOCATION:Cell membrane; Multi-pass membrane protein By similarity. Cell junction › synapse › postsynaptic cell membrane By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities By similarity. PTM:NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity By similarity. SEQUENCE SIMILARITIES:Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification]
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on untreated and alkaline phosphatase treated mouse brain cell lysates.
Western Blot Analysis: 1:1,000-2,000 dilution of this antibody was used to detect NMDAR1 in untreated and alkaline phosphatase treated mouse brain cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Glutamate is a widely utilized excitatory neurotransmitter in mammalian brain. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate (KA) and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are referred to as non-NMDA receptors. Two distinct genes encode the NMDA receptor subunits, NR1 and NR2. The NR1 subunit is expressed in abundance in virtually all regions of the brain.
Product Information
Format
Unpurified
Control
Mouse brain cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
This Anti-phospho-NMDAR1(Ser889) Antibody, clone EPR2480Y, Rabbit is validated for use in WB, IH(P), IC, IP for the detection of phospho-NMDAR1(Ser889).
Key Applications
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected NMDAR1 in human brain tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:30 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Phospho-specific peptide corresponding to residues surrounding Ser889 of human NMDAR1.
Epitope
Phosphorylated Ser889
Clone
EPR2480Y
Host
Rabbit
Specificity
This antibody detects NMDAR1 phosphorylated at Ser889.
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.
FUNCTION:NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors By similarity. SUBUNIT STRUCTURE:Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ By similarity. SUBCELLULAR LOCATION:Cell membrane; Multi-pass membrane protein By similarity. Cell junction › synapse › postsynaptic cell membrane By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities By similarity. PTM:NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity By similarity. SEQUENCE SIMILARITIES:Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification]
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on untreated and alkaline phosphatase treated mouse brain cell lysates.
Western Blot Analysis: 1:1,000-2,000 dilution of this antibody was used to detect NMDAR1 in untreated and alkaline phosphatase treated mouse brain cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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