Met is a receptor protein kinase (RPTK) for a multifunctional cytokine called (HFG) which controls cell growth and motility. Met has unique features that distinguish it from other members of RPTK's.
Product Information
Format
Unpurified
Control
A431 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-Met (Tyr1349) using this Anti-phospho-Met (Tyr1349) Antibody, clone EP2367Y, Rabbit validated for use in WB, IH(P), IC & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Met in human breast carcinoma tissue.
Immunocytochemistry Analysis: A 1:50 -100 dilution from a previous lot worked in IC.
Immunoprecipitation: A 1:20 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Phospho-specific peptide corresponding to residues surrounding Tyr1349 of human Met.
Epitope
Phosphorylated Tyr1349
Clone
EP2367Y
Host
Rabbit
Specificity
This antibody detects Met phosphorylated at Tyr1349.
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
FUNCTION:Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE:Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 By similarity. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein. DOMAIN:The kinase domain is involved in SPSB1 binding. INVOLVEMENT IN DISEASE:Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance.
Genetic variations in MET may be associated with susceptibility to autism type 9 (AUTS9) [MIM:611015]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on untreated and alkaline phosphatase-treated A431 cell lysates.
Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect Met in A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Met is a receptor protein kinase (RPTK) for a multifunctional cytokine called (HFG) which controls cell growth and motility. Met has unique features that distinguish it from other members of RPTK's.
Product Information
Format
Unpurified
Control
A431 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-Met (Tyr1349) using this Anti-phospho-Met (Tyr1349) Antibody, clone EP2367Y, Rabbit validated for use in WB, IH(P), IC & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Met in human breast carcinoma tissue.
Immunocytochemistry Analysis: A 1:50 -100 dilution from a previous lot worked in IC.
Immunoprecipitation: A 1:20 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Phospho-specific peptide corresponding to residues surrounding Tyr1349 of human Met.
Epitope
Phosphorylated Tyr1349
Clone
EP2367Y
Host
Rabbit
Specificity
This antibody detects Met phosphorylated at Tyr1349.
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
FUNCTION:Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE:Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 By similarity. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. SUBCELLULAR LOCATION:Membrane; Single-pass type I membrane protein. DOMAIN:The kinase domain is involved in SPSB1 binding. INVOLVEMENT IN DISEASE:Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance.
Genetic variations in MET may be associated with susceptibility to autism type 9 (AUTS9) [MIM:611015]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on untreated and alkaline phosphatase-treated A431 cell lysates.
Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect Met in A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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