eIF4E is a eukaryotic translation initiation factor that is involved in the direction of ribosomes to the cap structure of mRNA's. There are two forms of eIF4E: a free form
(25 kDa) and as part of a multi-protein complex (eIF-4F). The free form seems to not be as plentiful, compared to the other initiation factors and acts as a rate limiting step of initiation.
Product Information
Format
Unpurified
Control
293 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Detect phospho-eIF4E (Ser209) using this Anti-phospho-eIF4E (Ser209) Antibody, clone EP2151Y, Rabbit validated for use in WB, IH(P), IC & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected eIF4E in human breast carcinoma tissue.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot worked in IC.
Immunoprecipitation: A 1:60 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues surrounding phosphorylated Ser209 of human eIF4E.
Epitope
Phosphorylated Ser209
Clone
EP2151Y
Host
Rabbit
Specificity
This antibody recognizes eIF4E phosphorylated at Ser209.
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).
FUNCTION:Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. SUBUNIT STRUCTURE:eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 and EIF4A2. Interacts with NGDN and PIWIL2 By similarity. Interacts with Lassa virus Z protein. PTM:Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. SEQUENCE SIMILARITIES:Belongs to the eukaryotic initiation factor 4E family. CAUTION:Was originally thought to be phosphorylated on Ser-53 ; this was later shown to be wrong
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on untreated and alkaline phosphatase-treated 293 cell lysates.
Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect eIF4E in AP-treated 293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
eIF4E is a eukaryotic translation initiation factor that is involved in the direction of ribosomes to the cap structure of mRNA's. There are two forms of eIF4E: a free form
(25 kDa) and as part of a multi-protein complex (eIF-4F). The free form seems to not be as plentiful, compared to the other initiation factors and acts as a rate limiting step of initiation.
Product Information
Format
Unpurified
Control
293 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Detect phospho-eIF4E (Ser209) using this Anti-phospho-eIF4E (Ser209) Antibody, clone EP2151Y, Rabbit validated for use in WB, IH(P), IC & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected eIF4E in human breast carcinoma tissue.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot worked in IC.
Immunoprecipitation: A 1:60 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues surrounding phosphorylated Ser209 of human eIF4E.
Epitope
Phosphorylated Ser209
Clone
EP2151Y
Host
Rabbit
Specificity
This antibody recognizes eIF4E phosphorylated at Ser209.
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).
FUNCTION:Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. SUBUNIT STRUCTURE:eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 and EIF4A2. Interacts with NGDN and PIWIL2 By similarity. Interacts with Lassa virus Z protein. PTM:Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. SEQUENCE SIMILARITIES:Belongs to the eukaryotic initiation factor 4E family. CAUTION:Was originally thought to be phosphorylated on Ser-53 ; this was later shown to be wrong
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on untreated and alkaline phosphatase-treated 293 cell lysates.
Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect eIF4E in AP-treated 293 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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