The matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, lupus, arthritis, periodontis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.
MMP2, also known as Gelatinase A, is a type IV collagenase that specifically cleaves type IV collagen, the major structural component of basement membranes. The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme.
Product Information
Format
Unpurified
Control
Recombinant protein
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-MMP-2 Antibody, clone EP1183Y, Rabbit is validated for use in WB for the detection of MMP-2.
Key Applications
Western Blotting
Biological Information
Immunogen
Synthetic peptide corresponding to residues on human MMP-2.
Clone
EP1183Y
Host
Rabbit
Specificity
This antibody detects both the pro- and active forms of MMP-2.
FUNCTION:In addition to gelatin and collagens, it cleaves KiSS1 at a Gly-|-Leu bond.
CATALYTIC ACTIVITY:Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.
COFACTOR:Binds 4 calcium ions per subunit.
Binds 2 zinc ions per subunit.
ENZYME REGULATION:Inhibited by histatin-3 1/24 (histatin-5).
SUBUNIT STRUCTURE:Ligand for integrin alpha-V/beta-3.
SUBCELLULAR LOCATION:Secreted › extracellular space › extracellular matrix Probable.
TISSUE SPECIFICITY:Produced by normal skin fibroblasts.
DOMAIN:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM:The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3).
INVOLVEMENT IN DISEASE:Defects in MMP2 are the cause of Torg-Winchester syndrome [MIM:259600]; also called multicentric osteolysis nodulosis and arthropathy (MONA). Torg-Winchester syndrome is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
SEQUENCE SIMILARITIES:Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot using Anti-MMP-2.
Western Blot Analysis: 1:1000 dilution of this antibody was used to detect MMP-2.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
The matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, lupus, arthritis, periodontis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.
MMP2, also known as Gelatinase A, is a type IV collagenase that specifically cleaves type IV collagen, the major structural component of basement membranes. The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme.
Product Information
Format
Unpurified
Control
Recombinant protein
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-MMP-2 Antibody, clone EP1183Y, Rabbit is validated for use in WB for the detection of MMP-2.
Key Applications
Western Blotting
Biological Information
Immunogen
Synthetic peptide corresponding to residues on human MMP-2.
Clone
EP1183Y
Host
Rabbit
Specificity
This antibody detects both the pro- and active forms of MMP-2.
FUNCTION:In addition to gelatin and collagens, it cleaves KiSS1 at a Gly-|-Leu bond.
CATALYTIC ACTIVITY:Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.
COFACTOR:Binds 4 calcium ions per subunit.
Binds 2 zinc ions per subunit.
ENZYME REGULATION:Inhibited by histatin-3 1/24 (histatin-5).
SUBUNIT STRUCTURE:Ligand for integrin alpha-V/beta-3.
SUBCELLULAR LOCATION:Secreted › extracellular space › extracellular matrix Probable.
TISSUE SPECIFICITY:Produced by normal skin fibroblasts.
DOMAIN:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM:The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3).
INVOLVEMENT IN DISEASE:Defects in MMP2 are the cause of Torg-Winchester syndrome [MIM:259600]; also called multicentric osteolysis nodulosis and arthropathy (MONA). Torg-Winchester syndrome is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
SEQUENCE SIMILARITIES:Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot using Anti-MMP-2.
Western Blot Analysis: 1:1000 dilution of this antibody was used to detect MMP-2.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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