In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.
Product Information
Format
Unpurified
Control
SHSY5Y cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Musashi-1 (N-term) Antibody, clone EP1302, Rabbit is an antibody against Musashi-1 (N-term) for use in WB, IH(P), FC, IC & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Flow Cytometry
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Musashi-1 in human brain tissue.
Immunocytochemistry Analysis: A 1:250 - 500 dilution from a previous lot detected Musashi-1 in PC12 cells.
Flow Cytometry: A 1:40 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues on the N-terminus of human Musashi-1.
Epitope
N-terminus
Clone
EP1302
Host
Rabbit
Specificity
This antibody recognizes Musashi-1 at and around the N-terminus.
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
FUNCTION:RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity. SUBCELLULAR LOCATION:Cytoplasm By similarity. Nucleus By similarity. TISSUE SPECIFICITY:Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. DOMAIN:The first RNA recognition motif binds more strongly to RNA compared to the second one By similarity. SEQUENCE SIMILARITIES:Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on SHSY5Y cell lysates.
Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect Musashi-1 in SHSY5Y cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.
Product Information
Format
Unpurified
Control
SHSY5Y cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Musashi-1 (N-term) Antibody, clone EP1302, Rabbit is an antibody against Musashi-1 (N-term) for use in WB, IH(P), FC, IC & IP.
Key Applications
Immunoprecipitation
Western Blotting
Immunohistochemistry (Paraffin)
Flow Cytometry
Immunocytochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Musashi-1 in human brain tissue.
Immunocytochemistry Analysis: A 1:250 - 500 dilution from a previous lot detected Musashi-1 in PC12 cells.
Flow Cytometry: A 1:40 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues on the N-terminus of human Musashi-1.
Epitope
N-terminus
Clone
EP1302
Host
Rabbit
Specificity
This antibody recognizes Musashi-1 at and around the N-terminus.
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
FUNCTION:RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity. SUBCELLULAR LOCATION:Cytoplasm By similarity. Nucleus By similarity. TISSUE SPECIFICITY:Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. DOMAIN:The first RNA recognition motif binds more strongly to RNA compared to the second one By similarity. SEQUENCE SIMILARITIES:Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on SHSY5Y cell lysates.
Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect Musashi-1 in SHSY5Y cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
