Anti-Aurora C (NT) Antibody, clone EP1011Y, rabbit monoclonal
Background Information
Aurora A, B, and C, are members of the serine/threonine kinase family. These have been identified as important regulators of the mitotic cell division process. Aurora B, specifically, is involved in the regulation of centrosome function, bipolar spindle assembly, and chromosome segregation. Aurora-C is a chromosomal passenger protein that form complexes with Aurora B and INCENP (which binds and activates Aurora C in vivo and in vitro).
Product Information
Format
Unpurified
Control
HepG2 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Anti-Aurora C (N-term) Antibody, clone EP1011Y, Rabbit detects level of Aurora C (N-term) & has been published & validated for use in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the N-terminus of human Aurora C.
Epitope
N-terminus
Clone
EP1011Y
Host
Rabbit
Specificity
This antibody recognizes Aurora C at and around the N-terminus.
This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants.
FUNCTION:May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. CATALYTIC ACTIVITY:ATP + a protein = ADP + a phosphoprotein. COFACTOR:Magnesium. SUBUNIT STRUCTURE:Interacts with TACC1. Associates with RACGAP1 during M phase. Component of the CPC at least composed of BIRC5/survivin CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with CDCA1 and NDC80. Interacts with EVI5. SUBCELLULAR LOCATION:Nucleus. Centromere. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. TISSUE SPECIFICITY:High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. INVOLVEMENT IN DISEASE:Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on HepG2 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect Aurora C in HepG2 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
WB
Rb
Unpurified
Monoclonal Antibody
Description
Catalogue Number
04-1038
Description
Anti-Aurora C (NT) Antibody, clone EP1011Y, rabbit monoclonal
Background Information
Aurora A, B, and C, are members of the serine/threonine kinase family. These have been identified as important regulators of the mitotic cell division process. Aurora B, specifically, is involved in the regulation of centrosome function, bipolar spindle assembly, and chromosome segregation. Aurora-C is a chromosomal passenger protein that form complexes with Aurora B and INCENP (which binds and activates Aurora C in vivo and in vitro).
Product Information
Format
Unpurified
Control
HepG2 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Anti-Aurora C (N-term) Antibody, clone EP1011Y, Rabbit detects level of Aurora C (N-term) & has been published & validated for use in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
Synthetic peptide corresponding to residues near the N-terminus of human Aurora C.
Epitope
N-terminus
Clone
EP1011Y
Host
Rabbit
Specificity
This antibody recognizes Aurora C at and around the N-terminus.
This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants.
FUNCTION:May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. CATALYTIC ACTIVITY:ATP + a protein = ADP + a phosphoprotein. COFACTOR:Magnesium. SUBUNIT STRUCTURE:Interacts with TACC1. Associates with RACGAP1 during M phase. Component of the CPC at least composed of BIRC5/survivin CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with CDCA1 and NDC80. Interacts with EVI5. SUBCELLULAR LOCATION:Nucleus. Centromere. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body. TISSUE SPECIFICITY:High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. INVOLVEMENT IN DISEASE:Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on HepG2 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect Aurora C in HepG2 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
