STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases. In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT. Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand. After dimerization, the STATs accumulate in the nucleus by way of nuclear transport. STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production. The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.
Product Information
Format
Unpurified
Control
A431 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications
Flow Cytometry
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Stat5α in human squamous lung carcinoma tissue.
Immunocytochemistry Analysis: A 1:100 dilution from a previous lot was used in IC.
Flow Cytometry: A 1:10 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:80 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues in C-terminus of human STAT5α.
Epitope
C-terminus
Clone
E289
Host
Rabbit
Specificity
The antibody does not cross-react with other STAT family members.
FUNCTION:Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. SUBUNIT STRUCTURE:Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 By similarity. Interacts with NCOA1 and SOCS7. SUBCELLULAR LOCATION:Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation. PTM:Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity By similarity. SEQUENCE SIMILARITIES:Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on A431 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect STAT5α in A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases. In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT. Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand. After dimerization, the STATs accumulate in the nucleus by way of nuclear transport. STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production. The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.
Product Information
Format
Unpurified
Control
A431 cell lysate
Presentation
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applications
Application
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications
Flow Cytometry
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Stat5α in human squamous lung carcinoma tissue.
Immunocytochemistry Analysis: A 1:100 dilution from a previous lot was used in IC.
Flow Cytometry: A 1:10 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:80 dilution from a previous lot was used in IP.
Biological Information
Immunogen
Synthetic peptide corresponding to residues in C-terminus of human STAT5α.
Epitope
C-terminus
Clone
E289
Host
Rabbit
Specificity
The antibody does not cross-react with other STAT family members.
FUNCTION:Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. SUBUNIT STRUCTURE:Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 By similarity. Interacts with NCOA1 and SOCS7. SUBCELLULAR LOCATION:Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation. PTM:Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity By similarity. SEQUENCE SIMILARITIES:Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on A431 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect STAT5α in A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
