SOCS1, also known as Suppressor of cytokine signaling 1, or JAK-binding protein (JAB), STAT-induced STAT inhibitor 1 (SSI-1), or Tec-interacting protein 3 (TIP3), and encoded by the gene SOCS1/CISH1/SSI1/TIP3, is involved in the negative regulation of cytokines signaling that is mediated by several kinases including JAKs, TEC, KIT, GRB2 and VAV kinases. SOCS1 inhibits kinase activity via binding to the target kinase. SOCS1 also appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF), and SOCS1regulates interferon-gamma mediated sensory neuron survival and is a negative regulator in IGF1R signaling pathway. Finally, SOCS1 forms the substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. SOCS1 is localized to the nucleus and cytoplasmic vesicles. SOCS1 is expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
This Anti-SOCS1 Antibody, clone 4H1 (Ascites Free) is validated for use in western blotting & IP for the detection of SOCS1.
Key Applications
Immunoprecipitation
Western Blotting
Application Notes
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated SOCS1 in cell lysates.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to mouse SOCS1.
Clone
4H1
Concentration
1 mg/mL
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Mouse based on 100% sequence homology.
Evaluated by Western Blotting in KG-1 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SOCS1 in 10 µg of KG-1 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Description
Catalogue Number
04-002-C
Trade Name
Upstate
Description
Anti-SOCS1 Antibody, clone 4H1 (Ascites Free)
Background Information
SOCS1, also known as Suppressor of cytokine signaling 1, or JAK-binding protein (JAB), STAT-induced STAT inhibitor 1 (SSI-1), or Tec-interacting protein 3 (TIP3), and encoded by the gene SOCS1/CISH1/SSI1/TIP3, is involved in the negative regulation of cytokines signaling that is mediated by several kinases including JAKs, TEC, KIT, GRB2 and VAV kinases. SOCS1 inhibits kinase activity via binding to the target kinase. SOCS1 also appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF), and SOCS1regulates interferon-gamma mediated sensory neuron survival and is a negative regulator in IGF1R signaling pathway. Finally, SOCS1 forms the substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. SOCS1 is localized to the nucleus and cytoplasmic vesicles. SOCS1 is expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
This Anti-SOCS1 Antibody, clone 4H1 (Ascites Free) is validated for use in western blotting & IP for the detection of SOCS1.
Key Applications
Immunoprecipitation
Western Blotting
Application Notes
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated SOCS1 in cell lysates.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to mouse SOCS1.
Clone
4H1
Concentration
1 mg/mL
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Mouse based on 100% sequence homology.
Evaluated by Western Blotting in KG-1 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SOCS1 in 10 µg of KG-1 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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